Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fiore, A.; DallOsto, L.; Cazzaniga, S.; Diretto, G.; Giuliano, G.; Bassi, R.
    A quadruple mutant of Arabidopsis reveals a beta-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination of the PSI/PSII ratio (2012), BMC Plant Biol., 12, 50.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.158 genes lut1 and lut5, real-time PCR expression analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.158 additional information construction of the triple chy1chy2lut5 mutant, which is almost completely depleted in beta-xanthophylls. Quadruple chy1chy2lut2lut5 mutant, additionally carrying the lut2 mutation (affecting lycopene epsilon-cyclase) lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to chy1chy2lut5 mutant. Leaf carotenoid contents of wild-type and mutant enzymes, overview Arabidopsis thaliana
1.14.15.24 additional information mutants chy1chy2lut5 and chy1chy2lut2lut5 show almost no expression of LUT5 and strongly increased expression of LUT1 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.158 Fe2+ a heme-containing cytochrome P450 enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.158 alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.158 beta-carotene + reduced cytochrome P-450 + O2 Arabidopsis thaliana
-
 zeaxanthin + oxidized cytochrome P-450 + H2O
-
 ?
1.14.14.158 additional information Arabidopsis thaliana LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene ?
-
 ?
1.14.15.24 alpha-carotene + reduced acceptor + H+ + O2 Arabidopsis thaliana
-
 lutein + acceptor + H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Arabidopsis thaliana LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.158 Arabidopsis thaliana Q6TBX7 gene lut1
-
1.14.14.158 Arabidopsis thaliana Q93VK5 gene lut5
-
1.14.15.24 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.158 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.158 alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.158 beta-carotene + reduced cytochrome P-450 + O2
-
 Arabidopsis thaliana zeaxanthin + oxidized cytochrome P-450 + H2O
-
 ?
1.14.14.158 additional information LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene Arabidopsis thaliana ?
-
 ?
1.14.15.24 alpha-carotene + reduced acceptor + H+ + O2
-
 Arabidopsis thaliana lutein + acceptor + H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene Arabidopsis thaliana zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.158 CYP97a3
-
 Arabidopsis thaliana
1.14.14.158 CYP97C1
-
 Arabidopsis thaliana
1.14.14.158 LUT1
-
 Arabidopsis thaliana
1.14.14.158 LUT1/CYP97C1
-
 Arabidopsis thaliana
1.14.14.158 LUT5
-
 Arabidopsis thaliana
1.14.14.158 LUT5/CYP97A3
-
 Arabidopsis thaliana
1.14.15.24 beta-carotene hydroxylase
-
 Arabidopsis thaliana
1.14.15.24 CYP97a3
-
 Arabidopsis thaliana
1.14.15.24 CYP97C1
-
 Arabidopsis thaliana
1.14.15.24 LUT1
-
 Arabidopsis thaliana
1.14.15.24 LUT5
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.158 cytochrome P450
-
 Arabidopsis thaliana
1.14.14.158 heme
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.14.158 malfunction quadruple chy1chy2lut2lut5 mutant lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to the chy1chy2lut5 mutant. Chy1chy2lut2lut5 mutant plants show an even stronger photosensitivity than mutant chy1chy2lut5, a complete lack of qE, the rapidly reversible component of non-photochemical quenching, and a peculiar organization of the pigment binding complexes into thylakoids. The chy1chy2lut2lut5 mutant is depleted in Lhcb subunits and is specifically affected in Photosystem I function, showing a deficiency in PSI-LHCI supercomplexes, phenotype, overview Arabidopsis thaliana
1.14.14.158 metabolism the first step in xanthophyll biosynthesis from alpha- and beta-carotene is the hydroxylation of epsilon- and beta-rings, performed by both non-heme iron oxygenases CHY1 and CHY2, and by P450 cytochromes, LUT1/CYP97C1 and LUT5/CYP97A3. CHY1, CHY2, LUT1/CYP97C1 and LUT5/CYP97A3 are the complete complement of carotene hydroxylases in Arabidopsis thaliana Arabidopsis thaliana
1.14.14.158 physiological function the enzyme is involved in xanthophyll biosynthesis, correlation between xanthophyll levels and the PSI-PSII ratio, xanthophylls are needed for normal level of Photosystem I and LHCII accumulation Arabidopsis thaliana