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Literature summary extracted from

  • Feng, Z.; Mu, W.; Jiang, B.
    Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO (2013), Biotechnol. Lett., 35, 719-724.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expressed in Escherichia coli BL21(DE3) cells Pseudothermotoga lettingae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.6 64
-
 D-psicose at pH 8.0 and 75°C Pseudothermotoga lettingae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.6 additional information 1 mM Co2+, Mn2+, Mg2+, Ni2+, Zn2+, Ca2+, Cu2+, and EDTA do not influence enzyme activity Pseudothermotoga lettingae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 16015
-
 2 * 16015, calculated from amino acid sequence Pseudothermotoga lettingae
5.3.1.6 16800
-
 2 * 16800, SDS-PAGE Pseudothermotoga lettingae
5.3.1.6 35200
-
 gel filtration Pseudothermotoga lettingae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate Pseudothermotoga lettingae
-
 D-ribulose 5-phosphate
-
 ?
5.3.1.6 D-ribose 5-phosphate Pseudothermotoga lettingae TMO
-
 D-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Pseudothermotoga lettingae
-
-
-
5.3.1.6 Pseudothermotoga lettingae TMO
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 Ni-affinity column chromatography Pseudothermotoga lettingae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.6 0.2
-
 after 9.1fold purification, at pH 8.0 and 75°C Pseudothermotoga lettingae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-psicose the enzyme convertes D-psicose to D-allose maximally at 75°C and pH 8.0 with a 32% conversion yield Pseudothermotoga lettingae D-allose
-
 ?
5.3.1.6 D-psicose the enzyme convertes D-psicose to D-allose maximally at 75°C and pH 8.0 with a 32% conversion yield Pseudothermotoga lettingae TMO D-allose
-
 ?
5.3.1.6 D-ribose 5-phosphate
-
 Pseudothermotoga lettingae D-ribulose 5-phosphate
-
 ?
5.3.1.6 D-ribose 5-phosphate
-
 Pseudothermotoga lettingae TMO D-ribulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 homodimer 2 * 16800, SDS-PAGE Pseudothermotoga lettingae
5.3.1.6 homodimer 2 * 16015, calculated from amino acid sequence Pseudothermotoga lettingae

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 RPI
-
 Pseudothermotoga lettingae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.6 75
-
-
 Pseudothermotoga lettingae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.6 75
-
 the half-life at 75°C is 3.3 h Pseudothermotoga lettingae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.6 0.116
-
 D-psicose at pH 8.0 and 75°C Pseudothermotoga lettingae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.6 8
-
-
 Pseudothermotoga lettingae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.6 6 9 more than 80% of its maximal activity is maintained at pH values from 6.0 to 9.0 Pseudothermotoga lettingae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.6 0.0018
-
 D-psicose at pH 8.0 and 75°C Pseudothermotoga lettingae