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Literature summary extracted from
- Characterization of a recombinant L-rhamnose isomerase from Dictyoglomus turgidum and its application for L-rhamnulose production (2013), Biotechnol. Lett., 35, 259-264.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.14 | expressed in Escherichia coli ER2566 cells | Dictyoglomus turgidum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.14 | 24.6 | - |
L-rhamnose | at pH 8.0 and 75°C | Dictyoglomus turgidum |  |
| 5.3.1.14 | 33.4 | - |
L-Lyxose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 36.8 | - |
L-Mannose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 54.5 | - |
D-ribose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 61.5 | - |
D-allose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 63 | - |
L-xylulose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 73.2 | - |
L-Fructose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.14 | Mn2+ | activates | Dictyoglomus turgidum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.14 | 46695 | - |
4 * 46695, calculated from amino acid sequence | Dictyoglomus turgidum |
| 5.3.1.14 | 47000 | - |
4 * 47000, His6-tagged enzyme, SDS-PAGE | Dictyoglomus turgidum |
| 5.3.1.14 | 185000 | - |
gel filtration | Dictyoglomus turgidum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.14 | L-Rhamnose | Dictyoglomus turgidum | best substrate, conversion yield of 43% | L-Rhamnulose | - |
? | |
| 5.3.1.14 | L-Rhamnose | Dictyoglomus turgidum DSM 6724 | best substrate, conversion yield of 43% | L-Rhamnulose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.14 | Dictyoglomus turgidum | B8E1U6 | - |
- |
| 5.3.1.14 | Dictyoglomus turgidum DSM 6724 | B8E1U6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.14 | His-Trap HP column chromatography, and gel filtration | Dictyoglomus turgidum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.14 | D-allose | - |
Dictyoglomus turgidum | D-psicose | - |
? | |
| 5.3.1.14 | D-allose | - |
Dictyoglomus turgidum DSM 6724 | D-psicose | - |
? | |
| 5.3.1.14 | D-ribose | worst substrate | Dictyoglomus turgidum | D-ribulose | - |
? | |
| 5.3.1.14 | D-ribose | worst substrate | Dictyoglomus turgidum DSM 6724 | D-ribulose | - |
? | |
| 5.3.1.14 | L-fructose | - |
Dictyoglomus turgidum | L-mannose | - |
? | |
| 5.3.1.14 | L-lyxose | second best substrate | Dictyoglomus turgidum | L-xylulose | - |
? | |
| 5.3.1.14 | L-lyxose | second best substrate | Dictyoglomus turgidum DSM 6724 | L-xylulose | - |
? | |
| 5.3.1.14 | L-Mannose | - |
Dictyoglomus turgidum | L-Fructose | - |
? | |
| 5.3.1.14 | L-Mannose | - |
Dictyoglomus turgidum DSM 6724 | L-Fructose | - |
? | |
| 5.3.1.14 | L-Rhamnose | best substrate, conversion yield of 43% | Dictyoglomus turgidum | L-Rhamnulose | - |
? | |
| 5.3.1.14 | L-Rhamnose | best substrate, conversion yield of 43% | Dictyoglomus turgidum DSM 6724 | L-Rhamnulose | - |
? | |
| 5.3.1.14 | L-xylulose | - |
Dictyoglomus turgidum | L-lyxose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.14 | homotetramer | 4 * 46695, calculated from amino acid sequence | Dictyoglomus turgidum |
| 5.3.1.14 | homotetramer | 4 * 47000, His6-tagged enzyme, SDS-PAGE | Dictyoglomus turgidum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.14 | 75 | - |
maximum activities of both the free and immobilized enzymes for L-rhamnose isomerization | Dictyoglomus turgidum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.14 | 75 | - |
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+ | Dictyoglomus turgidum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.14 | 49 | - |
D-ribose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 81 | - |
D-allose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 116 | - |
L-Fructose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 130 | - |
L-xylulose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 155 | - |
L-Mannose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 195 | - |
L-rhamnose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 200 | - |
L-Lyxose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.14 | 8 | - |
maximum activities of both the free and immobilized enzymes for L-rhamnose isomerization | Dictyoglomus turgidum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.14 | 8 | - |
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+ | Dictyoglomus turgidum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.14 | 0.9 | - |
D-ribose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 1.3 | - |
D-allose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 1.6 | - |
L-Fructose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 2.1 | - |
L-xylulose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 4.2 | - |
L-Mannose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 6 | - |
L-Lyxose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.14 | 7.9 | - |
L-rhamnose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
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