Any feedback?
Literature summary extracted from
- Characterization of a recombinant cellobiose 2-epimerase from Dictyoglomus turgidum that epimerizes and isomerizes beta-1,4- and alpha-1,4-gluco-oligosaccharides (2012), Biotechnol. Lett., 34, 2061-2068.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.11 | expression of His-tagged enzyme in Escherichia coli | Dictyoglomus turgidum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 75.8 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum |  |
| 5.1.3.11 | 79.6 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 47000 | - |
recombinant His-tagged enzyme, gel filtration | Dictyoglomus turgidum |
| 5.1.3.11 | 47200 | - |
1 * 47000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 47200, about, recombinant His-tagged enzyme, sequence calculation | Dictyoglomus turgidum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.11 | cellobiose | Dictyoglomus turgidum | - |
D-glucosyl-D-mannose | - |
? | |
| 5.1.3.11 | cellobiose | Dictyoglomus turgidum DSM 6724 | - |
D-glucosyl-D-mannose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.11 | Dictyoglomus turgidum | - |
- |
- |
| 5.1.3.11 | Dictyoglomus turgidum DSM 6724 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.11 | recombinant His-tagged enzyme 10fold from Escherichia coli by heat treatment and metal affinity chromatography | Dictyoglomus turgidum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 14.2 | - |
purified recombinant His-tagged enzyme, substrate lactose, pH 7.0, 70°C | Dictyoglomus turgidum |
| 5.1.3.11 | 16.8 | 24.4 | purified recombinant His-tagged enzyme, substrate cellolbiose, pH 7.0, 70°C | Dictyoglomus turgidum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.11 | cellobiose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| 5.1.3.11 | cellobiose | - |
Dictyoglomus turgidum DSM 6724 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| 5.1.3.11 | cellobiose | - |
Dictyoglomus turgidum | D-glucosyl-D-mannose | - |
? | |
| 5.1.3.11 | cellobiose | - |
Dictyoglomus turgidum DSM 6724 | D-glucosyl-D-mannose | - |
? | |
| 5.1.3.11 | cellotriose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
| 5.1.3.11 | D-glucose | - |
Dictyoglomus turgidum | D-mannose | - |
? | |
| 5.1.3.11 | D-glucose | - |
Dictyoglomus turgidum DSM 6724 | D-mannose | - |
? | |
| 5.1.3.11 | lactose | - |
Dictyoglomus turgidum | epilactose | - |
? | |
| 5.1.3.11 | lactose | - |
Dictyoglomus turgidum DSM 6724 | epilactose | - |
? | |
| 5.1.3.11 | lactose | - |
Dictyoglomus turgidum | lactulose | - |
? | |
| 5.1.3.11 | maltose | - |
Dictyoglomus turgidum | 4-O-alpha-D-glucopyranosyl-D-mannose | - |
? | |
| 5.1.3.11 | maltotriose | - |
Dictyoglomus turgidum | 4-O-alpha-D-glucopyranosyl-4-O-alpha-D-glucopyranosyl-D-mannose | - |
? | |
| 5.1.3.11 | additional information | the enzyme catalyzes not only epimerization but also isomerization for beta-1,4- and alpha-1,4-gluco-oligosaccharides. No activity with trehalose, sophorose, laminaribiose, gentiobiose, or xylobiose | Dictyoglomus turgidum | ? | - |
? | |
| 5.1.3.11 | additional information | the enzyme catalyzes not only epimerization but also isomerization for beta-1,4- and alpha-1,4-gluco-oligosaccharides. No activity with trehalose, sophorose, laminaribiose, gentiobiose, or xylobiose | Dictyoglomus turgidum DSM 6724 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | monomer | 1 * 47000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 47200, about, recombinant His-tagged enzyme, sequence calculation | Dictyoglomus turgidum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | cellobiose 2-epimerase | - |
Dictyoglomus turgidum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 70 | - |
- |
Dictyoglomus turgidum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 65 | 85 | purified recombinant His-tagged enzyme, thermal inactivation of the cellobiose 2-epimerase follows first-order kinetics and the half-lives of the enzyme at 65°C, 70°C, 75°C, 80°C, and 85°C are 79 h, 55 h, 35 h, 20 h, and 4.8 h, respectively | Dictyoglomus turgidum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 89.4 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
| 5.1.3.11 | 169.1 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.11 | 7 | - |
- |
Dictyoglomus turgidum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.3.11 | evolution | structure, active site, and reaction mechanism of cellobiose 2-epimerase are similar to those of N-acyl-D-glucosamine 2-epimerase | Dictyoglomus turgidum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.11 | 0.019 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
| 5.1.3.11 | 0.037 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
