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Literature summary extracted from

  • Zhang, L.; Mu, W.; Jiang, B.; Zhang, T.
    Characterization of D-tagatose-3-epimerase from Rhodobacter sphaeroides that converts D-fructose into D-psicose (2009), Biotechnol. Lett., 31, 857-862.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.31 expressed in Escherichia coli Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.31 Cu2+
-
 Cereibacter sphaeroides
5.1.3.31 Zn2+
-
 Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.31 Mn2+ required, strongly enhances activity Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.31 32000
-
 2 * 32000, SDS-PAGE Cereibacter sphaeroides
5.1.3.31 64000
-
 gel filtration Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.31 Cereibacter sphaeroides C1KKR1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.31 D-fructose specificity is highest with D-fructose and decreases for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose. The equilibrium ratio between D-psicose and D-fructose is 23:77 after 24 h at 40°C Cereibacter sphaeroides D-psicose
-
 r
5.1.3.31 D-ribulose specificity is highest with D-fructose and decreases for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose Cereibacter sphaeroides D-xylulose
-
 ?
5.1.3.31 D-tagatose specificity is highest with D-fructose and decreases for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose Cereibacter sphaeroides D-sorbose
-
 r
5.1.3.31 additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate Cereibacter sphaeroides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.31 dimer 2 * 32000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.31 40
-
-
 Cereibacter sphaeroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.31 30 50 30°C: about 70% of maximal activity, 50°C: about 70% of maximal activity Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.31 40
-
 4 h, less than 10% loss of activity Cereibacter sphaeroides
5.1.3.31 45
-
 4 h, about 65% loss of activity Cereibacter sphaeroides
5.1.3.31 50
-
 1 h, about 50% loss of activity Cereibacter sphaeroides
5.1.3.31 55
-
 30 min, about 80% loss of activity Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.31 9
-
-
 Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.31 8 10 pH 8.0: about 75% of maximal activity, pH 10.0: about 70% of maximal activity Cereibacter sphaeroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.31 6
-
 4°C, 1 h, 65% loss of activity Cereibacter sphaeroides
5.1.3.31 7
-
 4°C, 1 h, 25% loss of activity Cereibacter sphaeroides
5.1.3.31 8 10 4°C, 1 h, stable Cereibacter sphaeroides
5.1.3.31 11
-
 4°C, 1 h, 30% loss of activity Cereibacter sphaeroides