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Literature summary extracted from

  • Zhang, J.; Lu, X.; Li, J.J.
    Conversion of fatty aldehydes into alk (a/e)nes by in vitro reconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system (2013), Biotechnol. Biofuels, 6, 86.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.5 cloning of gene Synpcc7942_159 from strain PCC7942, overexpression of N-terminally His-tagged protein in Escherichia coli strain BL21(DE3) Synechococcus elongatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.5 0.244
-
 n-Heptanal pH 7.2, 22°C, recombinant enzyme with Fd/FNR system Synechococcus elongatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.5 26400
-
 x * 26400, recombinant N-terminally His-tagged enzyme, SDS-PAGE Synechococcus elongatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.5 long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechococcus elongatus
-
 alkane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechococcus elongatus PCC 7942
-
 alkane + formate + H2O + 2 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.5 Synechococcus elongatus Q54764
-
-
4.1.99.5 Synechococcus elongatus PCC 7942 Q54764
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.5 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of the tag by thrombin, and gel filtration Synechococcus elongatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.5 hexadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus pentadecane + formate + H2O + 2 NAD(P)+
-
 ?
4.1.99.5 hexadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus PCC 7942 pentadecane + formate + H2O + 2 NAD(P)+
-
 ?
4.1.99.5 long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
 Synechococcus elongatus alkane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
 Synechococcus elongatus PCC 7942 alkane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 additional information the endogenous electron transfer system works more effectively than the heterologous and chemical ones, e.g. phenazine methosulfate or 1-methyoxy-5-methylphenazinium methylsulfate and NADH, overview Synechococcus elongatus ?
-
 ?
4.1.99.5 additional information the endogenous electron transfer system works more effectively than the heterologous and chemical ones, e.g. phenazine methosulfate or 1-methyoxy-5-methylphenazinium methylsulfate and NADH, overview Synechococcus elongatus PCC 7942 ?
-
 ?
4.1.99.5 n-heptanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus n-hexane + formate + H2O + 2 NAD(P)+
-
 ?
4.1.99.5 n-heptanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus PCC 7942 n-hexane + formate + H2O + 2 NAD(P)+
-
 ?
4.1.99.5 octadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus heptadecane + formate + H2O + 2 NAD(P)+
-
 ?
4.1.99.5 octadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus PCC 7942 heptadecane + formate + H2O + 2 NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.5 ? x * 26400, recombinant N-terminally His-tagged enzyme, SDS-PAGE Synechococcus elongatus

Synonyms

EC Number Synonyms Comment Organism
4.1.99.5 ADO
-
 Synechococcus elongatus
4.1.99.5 aldehyde decarbonylase UniProt Synechococcus elongatus
4.1.99.5 aldehyde-deformylating oxygenase
-
 Synechococcus elongatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.5 22
-
 assay at room temperature Synechococcus elongatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.5 0.0073
-
 n-Heptanal pH 7.2, 22°C, recombinant enzyme with Fd/FNR system Synechococcus elongatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.5 7.2
-
 assay at Synechococcus elongatus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.5 additional information ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase: ADO is selective against ferredoxin and the interaction between ferredoxin and ferredoxin-NADP+ reductase is very important for efficient electron transfer and ADO activity Synechococcus elongatus
4.1.99.5 NADH
-
 Synechococcus elongatus
4.1.99.5 NADPH physiological cofactor Synechococcus elongatus