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Literature summary extracted from

  • Nakagawa, S.; Kojima, Y.; Sekino, K.; Yamato, S.
    Effect of polyphenols on 3-hydroxy-3-methylglutaryl-coenzyme A lyase activity in human hepatoma HepG2 cell extracts (2013), Biol. Pharm. Bull., 36, 1902-1906.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.4 daidzein slight activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.4 (-)-epicatechin gallate a mixed-type noncompetitive inhibitor Homo sapiens
4.1.3.4 (-)-epigallocatechin a mixed-type noncompetitive inhibitor Homo sapiens
4.1.3.4 (-)-epigallocatechin gallate a mixed-type noncompetitive inhibitor, contribution of the gallyl moiety, which forms a hexa-coordination complex with the Mg2+ ion, to enzymatic inhibition Homo sapiens
4.1.3.4 gallic acid a noncompetitive inhibitor Homo sapiens
4.1.3.4 additional information no inhibition by daidzein, hesperetin, naringenin, (+)-catechin, and (-)-epicatechin Homo sapiens
4.1.3.4 pyrogallol a mixed-type noncompetitive inhibitor Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.4 Hep-G2 cell
-
 Homo sapiens
-
4.1.3.4 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA
-
 Homo sapiens Acetyl-CoA + acetoacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.4 3-hydroxy-3-methylglutaryl-coenzyme A lyase
-
 Homo sapiens
4.1.3.4 HMG-CoA lyase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.4 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 7.5
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.4 0.0028
-
 (-)-epigallocatechin gallate pH 7.5, 37°C Homo sapiens
4.1.3.4 0.0074
-
 pyrogallol pH 7.5, 37°C Homo sapiens
4.1.3.4 0.018
-
 gallic acid pH 7.5, 37°C Homo sapiens
4.1.3.4 0.0299
-
 (-)-epigallocatechin pH 7.5, 37°C Homo sapiens
4.1.3.4 0.0441
-
 (-)-epicatechin gallate pH 7.5, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.3.4 0.0424
-
 pH 7.5, 37°C Homo sapiens (-)-epigallocatechin gallate
4.1.3.4 0.0444
-
 pH 7.5, 37°C Homo sapiens gallic acid
4.1.3.4 0.0575
-
 pH 7.5, 37°C Homo sapiens (-)-epigallocatechin
4.1.3.4 0.0615
-
 pH 7.5, 37°C Homo sapiens pyrogallol
4.1.3.4 0.1612
-
 pH 7.5, 37°C Homo sapiens (-)-epicatechin gallate

General Information

EC Number General Information Comment Organism
4.1.3.4 malfunction inhibition of this enzyme results in reduced acetoacetate production and impaired ketoacidosis. High levels of secreted ketone bodies (acetoacetate and 3beta-hydroxybutyrate) lower the pH of blood and urine, resulting in ketoacidosis Homo sapiens
4.1.3.4 physiological function the enzyme is a rate-limiting enzyme in hepatic cells catalyzing the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetate Homo sapiens