Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Del Giudice, I.; Limauro, D.; Pedone, E.; Bartolucci, S.; Fiorentino, G.
    A novel arsenate reductase from the bacterium Thermus thermophilus HB27: its role in arsenic detoxification (2013), Biochim. Biophys. Acta, 1834, 2071-2079.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.4 expression in Escherichia coli Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.20.4.4 C7S complete loss of activity Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.4.4 0.635
-
 arsenate pH 7.0, 60°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.4 16957
-
 1 * 16957, calculated, 1 * 17000, SDS-PAGE Thermus thermophilus
1.20.4.4 17000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.4 Thermus thermophilus Q72HI5
-
-
1.20.4.4 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72HI5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.4.4 recombinant protein Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.4 arsenate + thioredoxin
-
 Thermus thermophilus arsenite + thioredoxin disulfide + H2O
-
 ?
1.20.4.4 arsenate + thioredoxin
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 arsenite + thioredoxin disulfide + H2O
-
 ?
1.20.4.4 additional information enzyme additionally exhibits weak phosphatase activity Thermus thermophilus ?
-
 ?
1.20.4.4 additional information enzyme additionally exhibits weak phosphatase activity Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.4.4 monomer 1 * 16957, calculated, 1 * 17000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.20.4.4 ARS
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.20.4.4 0.75
-
 arsenate pH 7.0, 60°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.4.4 thioredoxin
-
 Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.20.4.4 Thermus thermophilus calculated
-
 6.5

Expression

EC Number Organism Comment Expression
1.20.4.4 Thermus thermophilus expression is 4fold increased in presence of arsenate in the growth medium up