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Literature summary extracted from

  • Varvas, K.; Kasvandik, S.; Hansen, K.; Jaerving, I.; Morell, I.; Samel, N.
    Structural and catalytic insights into the algal prostaglandin H synthase reveal atypical features of the first non-animal cyclooxygenase (2013), Biochim. Biophys. Acta, 1831, 863-871.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.1 PGHS DNA and amiino acid sequence determination and analysis, sequence comparisons Coccotylus truncatus
1.14.99.1 PGHS DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)RP Agarophyton vermiculophyllum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.1 additional information algal PGHS is not inhibited by non-steroidal anti-inflammatory drugs that inhibit the mammalian enzymes Agarophyton vermiculophyllum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.1 additional information
-
additional information Michaelis-Menten kinetics Agarophyton vermiculophyllum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.1 membrane bound Agarophyton vermiculophyllum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.1 Fe2+ ferric heme enzyme Agarophyton vermiculophyllum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.1 63500
-
4 * 63500, recombinant His-tagged enzyme, SDS-PAGE Agarophyton vermiculophyllum
1.14.99.1 330000
-
about, recombinant His-tagged enzyme, gel filtration Agarophyton vermiculophyllum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.1 arachidonate + AH2 + 2 O2 Agarophyton vermiculophyllum
-
prostaglandin G2 + A + ?
-
?
1.14.99.1 arachidonate + AH2 + 2 O2 Agarophyton vermiculophyllum
-
prostaglandin H2 + A + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.1 Agarophyton vermiculophyllum I6VVK9 collected from the coast of Kanagawa Prefecture in Tokyo Bay
-
1.14.99.1 Coccotylus truncatus I6VCP8 collected on the coast of the Baltic Sea in Kassari Bay
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.1 recombinant His-tagged enzyme 138fold from Escherichia coli strain BL21(DE3)RP membranes by nickel affinity chromatography, and anion exchange chromatography Agarophyton vermiculophyllum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.1 arachidonate + AH2 + 2 O2
-
Agarophyton vermiculophyllum prostaglandin G2 + A + ?
-
?
1.14.99.1 arachidonate + AH2 + 2 O2
-
Agarophyton vermiculophyllum prostaglandin H2 + A + H2O
-
?
1.14.99.1 arachidonate + reduced N,N,N',N'-tetramethylphenylenediamine + 2 O2
-
Agarophyton vermiculophyllum prostaglandin G2 + oxidized N,N,N',N'-tetramethylphenylenediamine + ?
-
?
1.14.99.1 arachidonate + reduced N,N,N',N'-tetramethylphenylenediamine + 2 O2
-
Agarophyton vermiculophyllum prostaglandin H2 + oxidized N,N,N',N'-tetramethylphenylenediamine + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.99.1 More structure comparisons, overview Agarophyton vermiculophyllum
1.14.99.1 tetramer 4 * 63500, recombinant His-tagged enzyme, SDS-PAGE Agarophyton vermiculophyllum

Synonyms

EC Number Synonyms Comment Organism
1.14.99.1 PGHS
-
Agarophyton vermiculophyllum
1.14.99.1 PGHS
-
Coccotylus truncatus
1.14.99.1 prostaglandin H synthase
-
Agarophyton vermiculophyllum
1.14.99.1 prostaglandin H synthase
-
Coccotylus truncatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.1 25
-
cyclooxygenase activity assay at Agarophyton vermiculophyllum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.1 8
-
cyclooxygenase activity assay at Agarophyton vermiculophyllum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.1 heme ferric heme, heme content of the purified recombinant GvPGHS determined by a pyridine-hemochromogen assay is 0.39 mol of heme/mol of GvPGHS monomer Agarophyton vermiculophyllum

General Information

EC Number General Information Comment Organism
1.14.99.1 evolution the algal PGHS lacks structural elements identified in all known animal PGHSs, such as epidermal growth factor-like domain and helix B in the membrane binding domain. The key residues of animal PGHS, like catalytic Tyr385 and heme liganding His388 are conserved in the algal enzyme, but the amino acid residues shown to be important for substrate binding and coordination, and the target residues for nonsteroidal anti-inflammatory drugs, Arg120, Tyr355, and Ser530, are not found at the appropriate positions in the algal sequences. The preferred substrate for the algal PGHS is arachidonic acid with cyclooxygenase reaction rate remarkably higher than values reported for mammalian PGHS isoforms Agarophyton vermiculophyllum