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Literature summary extracted from

  • Di Venere, A.; Horn, T.; Stehling, S.; Mei, G.; Masgrau, L.; Gonzalez-Lafont, A.; Kuehn, H.; Ivanov, I.
    Role of Arg403 for thermostability and catalytic activity of rabbit 12/15-lipoxygenase (2013), Biochim. Biophys. Acta, 1831, 1079-1088.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 expressed in Escherichia coli as a His-tagged fusion protein Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.31 R403L a loss of electrostatic interaction between Arg403 and negatively charged amino acid residues of alpha2-helix has only minor impact on protein folding, but partially destabilizes the tertiary structure of the enzyme. Arg403Leu exchange induces strong substrate inhibition. kcat/Km values strongly decreased for linoleic acid and methyl arachidonate but almost unchanged for arachidonic acid compared to wild-type Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.0082
-
 arachidonic acid pH 7.4, 20°C, wild-type Oryctolagus cuniculus
1.13.11.31 0.0109
-
 arachidonic acid pH 7.4, 20°C, mutant R403L Oryctolagus cuniculus
1.13.11.31 0.0114
-
 methyl arachidonate pH 7.4, 20°C, mutant R403L Oryctolagus cuniculus
1.13.11.31 0.0169
-
 linoleic acid pH 7.4, 20°C, wild-type Oryctolagus cuniculus
1.13.11.31 0.018
-
 methyl arachidonate pH 7.4, 20°C, wild-type Oryctolagus cuniculus
1.13.11.31 0.0281
-
 linoleic acid pH 7.4, 20°C, mutant R403L Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 using Ni-NTA chromatography Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonic acid + O2
-
 Oryctolagus cuniculus ?
-
 ?
1.13.11.31 linoleic acid + O2
-
 Oryctolagus cuniculus ?
-
 ?
1.13.11.31 methyl arachidonate + O2
-
 Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12/15-lipoxygenase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.31 20
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.4
-
 assay at Oryctolagus cuniculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.31 170
-
 methyl arachidonate pH 7.4, 20°C, mutant R403L Oryctolagus cuniculus
1.13.11.31 760
-
 methyl arachidonate pH 7.4, 20°C, wild-type Oryctolagus cuniculus
1.13.11.31 830
-
 linoleic acid pH 7.4, 20°C, mutant R403L Oryctolagus cuniculus
1.13.11.31 1410
-
 arachidonic acid pH 7.4, 20°C, wild-type Oryctolagus cuniculus
1.13.11.31 1950
-
 arachidonic acid pH 7.4, 20°C, mutant R403L Oryctolagus cuniculus
1.13.11.31 2650
-
 linoleic acid pH 7.4, 20°C, wild-type Oryctolagus cuniculus