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Literature summary extracted from
- Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics (2014), Biochemistry, 79, 54-61.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | 2-mercaptoethanol | 2 mM, 2fold increase in activity | Acidilobus saccharovorans |  |
| 3.5.1.19 | dithiothreitol | 2 mM, 2.1fold increase in activity | Acidilobus saccharovorans | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.B15 | expressed in Escherichia coli | Acidilobus saccharovorans |
| 3.5.1.19 | expressed in Escherichia coli | Acidilobus saccharovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.B15 | Zn2+ | - |
Acidilobus saccharovorans | |
| 3.5.1.19 | EDTA | 10 mM, 57% loss of activity | Acidilobus saccharovorans | |
| 3.5.1.19 | Fe3+ | 1 mM, 94% inhibition | Acidilobus saccharovorans | |
| 3.5.1.19 | NaCl | 500 mM, 12% inhibition | Acidilobus saccharovorans | |
| 3.5.1.19 | Zn2+ | 5 mM, complete inactivation | Acidilobus saccharovorans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.B15 | 0.41 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
| 3.5.1.19 | 0.18 | - |
nicotinamide | pH and temperature not specified in the publication | Acidilobus saccharovorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.B15 | Fe2+ | the enzyme contains Fe2+ in the active site | Acidilobus saccharovorans | |
| 3.5.1.19 | Fe2+ | the enzyme contains Fe2+ in the active site | Acidilobus saccharovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.B15 | 46400 | - |
- |
Acidilobus saccharovorans |
| 3.5.1.19 | 44600 | - |
- |
Acidilobus saccharovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.B15 | Acidilobus saccharovorans | D9Q1R5 | - |
- |
| 3.5.1.19 | Acidilobus saccharovorans | D9Q1R5 | - |
- |
| 3.5.1.19 | Acidilobus saccharovorans DSM 16705 | D9Q1R5 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.B15 | pyrazinamide + H2O | the enzyme also catalyzes the hydrolysis of nicotinamide | Acidilobus saccharovorans | pyrazinoic acid + NH3 | - |
? | |
| 3.5.1.B15 | pyrazinamide + H2O | the enzyme also catalyzes the hydrolysis of nicotinamide | Acidilobus saccharovorans DSM 16705 | pyrazinoic acid + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | the enzyme also catalyzes the hydrolysis of pyrazinamide | Acidilobus saccharovorans | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | the enzyme also catalyzes the hydrolysis of pyrazinamide | Acidilobus saccharovorans DSM 16705 | nicotinate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.B15 | homodimer | - |
Acidilobus saccharovorans |
| 3.5.1.19 | homodimer | - |
Acidilobus saccharovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.B15 | ASAC_0847 | locus name | Acidilobus saccharovorans |
| 3.5.1.19 | ASAC_0847 | locus name | Acidilobus saccharovorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.B15 | 90 | - |
- |
Acidilobus saccharovorans |
| 3.5.1.19 | 90 | - |
- |
Acidilobus saccharovorans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 65 | 98 | 65°C: about 50% of maximal activity, 98°C: about 80% of maximal activity | Acidilobus saccharovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.B15 | 60 | - |
t1/2: 180 min | Acidilobus saccharovorans |
| 3.5.1.B15 | 80 | - |
t1/2: 35 min | Acidilobus saccharovorans |
| 3.5.1.19 | 60 | - |
t1/2: 180 min | Acidilobus saccharovorans |
| 3.5.1.19 | 80 | - |
t1/2: 35 min | Acidilobus saccharovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.B15 | 135.7 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
| 3.5.1.19 | 76.9 | - |
nicotinamide | pH and temperature not specified in the publication | Acidilobus saccharovorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.B15 | 7.5 | - |
- |
Acidilobus saccharovorans |
| 3.5.1.19 | 7.5 | - |
- |
Acidilobus saccharovorans |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | physiological function | the enzyme is involved in the maintenance of NAD+ homeostasis and in the NAD+ salvage pathway of most prokaryotes | Acidilobus saccharovorans |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.B15 | 331 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
| 3.5.1.19 | 427 | - |
nicotinamide | pH and temperature not specified in the publication | Acidilobus saccharovorans | |
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