EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.185 | expression in Escherichia coli | Thermoplasma acidophilum |
2.7.1.186 | expression in Escherichia coli | Thermoplasma acidophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.185 | 0.097 | - |
(R)-mevalonate | pH 8.5, 55°C | Thermoplasma acidophilum | |
2.7.1.186 | additional information | - |
(R)-3-phosphomevalonate | pH 8.0, 60°C, it is not possible to accurately determine the Km for mevalonate-3-phosphate-5-kinase, however, because the substrate is not commercially available and the detection limit of the assay is 0.03 mM substrate. The enzyme is still at Vmax when it is assayed with 0.06 mM mevalonate 3-phosphate, indicating that the Km is well below 0.06 mM | Thermoplasma acidophilum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.185 | ATP + (R)-mevalonate | Thermoplasma acidophilum | mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum | ADP + (R)-3-phosphomevalonate | - |
? | |
2.7.1.186 | ATP + (R)-3-phosphomevalonate | Thermoplasma acidophilum | mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum | ADP + mevalonate 3,5-bisphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.185 | Thermoplasma acidophilum | Q9HIN1 | - |
- |
2.7.1.186 | Thermoplasma acidophilum | Q9HK44 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.185 | - |
Thermoplasma acidophilum |
2.7.1.186 | - |
Thermoplasma acidophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.185 | ATP + (R)-mevalonate | - |
Thermoplasma acidophilum | ADP + (R)-3-phosphomevalonate | - |
? | |
2.7.1.185 | ATP + (R)-mevalonate | mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum | Thermoplasma acidophilum | ADP + (R)-3-phosphomevalonate | - |
? | |
2.7.1.186 | ATP + (R)-3-phosphomevalonate | - |
Thermoplasma acidophilum | ADP + mevalonate 3,5-bisphosphate | - |
? | |
2.7.1.186 | ATP + (R)-3-phosphomevalonate | mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum | Thermoplasma acidophilum | ADP + mevalonate 3,5-bisphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.185 | Ta1305 | locus name | Thermoplasma acidophilum |
2.7.1.186 | Ta0762 | locus name | Thermoplasma acidophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.185 | 55 | - |
- |
Thermoplasma acidophilum |
2.7.1.186 | 60 | - |
- |
Thermoplasma acidophilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.185 | 40 | 60 | 40°C: about 55% of maximal activity, 60°C: about 95% of maximal activity | Thermoplasma acidophilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.185 | 60 | - |
1 h, enzyme retains more than 95% of its activity | Thermoplasma acidophilum |
2.7.1.185 | 70 | - |
1 h, about 75% loss of activity | Thermoplasma acidophilum |
2.7.1.185 | 75 | - |
1 h, complete loss of activity | Thermoplasma acidophilum |
2.7.1.186 | 60 | - |
1 h, enzyme retains more than 95% of its activity | Thermoplasma acidophilum |
2.7.1.186 | 70 | - |
1 h, about 75% loss of activity | Thermoplasma acidophilum |
2.7.1.186 | 80 | - |
1 h, complete loss of activity | Thermoplasma acidophilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.185 | 5 | - |
(R)-mevalonate | pH 8.5, 55°C | Thermoplasma acidophilum | |
2.7.1.186 | 9 | - |
(R)-3-phosphomevalonate | pH 8.0, 60°C | Thermoplasma acidophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.185 | 8.5 | - |
- |
Thermoplasma acidophilum |
2.7.1.186 | 6.5 | 9 | no significant pH dependence in the pH range of 6.5-9.0 | Thermoplasma acidophilum |
2.7.1.186 | 8 | - |
assay at | Thermoplasma acidophilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.185 | 7 | 9.5 | pH 7.0: about 75% of maximal activity, pH 9.5: about 85% of maximal activity | Thermoplasma acidophilum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.185 | physiological function | mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum | Thermoplasma acidophilum |
2.7.1.186 | physiological function | mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum | Thermoplasma acidophilum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.185 | 51.5 | - |
(R)-mevalonate | pH 8.5, 55°C | Thermoplasma acidophilum |