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Literature summary extracted from

  • Bartholow, T.G.; Sanford, B.L.; Cao, B.; Schmit, H.L.; Johnson, J.M.; Meitzner, J.; Bhattacharyya, S.; Musier-Forsyth, K.; Hati, S.
    Strictly conserved lysine of prolyl-tRNA synthetase editing domain facilitates binding and positioning of misacylated tRNAPro (2014), Biochemistry, 53, 1059-1068.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expressed in Escherichia coli SG13009 (pREP4) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 E303A the mutation results in 3fold decrease in L-proline activation. The mutant exhibits a small decrease in the aminoacylation efficiency Escherichia coli
6.1.1.15 E303D the mutation results in 3.1fold decrease in L-proline activation. The mutant exhibits a small decrease in the aminoacylation efficiency Escherichia coli
6.1.1.15 E303K the mutation results in 4.2fold decrease in L-proline activation. The mutant exhibits a small decrease in the aminoacylation efficiency Escherichia coli
6.1.1.15 K279E the mutation results in 2.7fold reduced L-proline activation. The mutant exhibits wild type aminoacylation efficiency Escherichia coli
6.1.1.15 K279E/E303K the mutant shows 2.3fold reduced L-proline activation. The mutant exhibits wild type aminoacylation efficiency Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.18
-
 L-proline wild type enzyme, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 0.19
-
 L-proline mutant enzyme K297E/E303K, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 0.199
-
 L-proline mutant enzyme E303D, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 0.216
-
 L-proline mutant enzyme E303A, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 0.22
-
 L-proline mutant enzyme K297E, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 0.3
-
 L-proline mutant enzyme E303K, at pH 7.5 and 22°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-alanine + tRNAPro Escherichia coli
-
 AMP + diphosphate + L-alanyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Escherichia coli
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 Talon cobalt affinity resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-alanine + tRNAPro
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
 Escherichia coli
6.1.1.15 ProRS
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 4.6
-
 L-proline mutant enzyme E303D, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 5.1
-
 L-proline mutant enzyme E303K, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 5.2
-
 L-proline mutant enzyme E303A, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 5.7
-
 L-proline mutant enzyme K297E, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 5.9
-
 L-proline mutant enzyme K297E/E303K, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 12.6
-
 L-proline wild type enzyme, at pH 7.5 and 22°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.15 17
-
 L-proline mutant enzyme E303K, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 23
-
 L-proline mutant enzyme E303D, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 24
-
 L-proline mutant enzyme E303A, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 26
-
 L-proline mutant enzyme K297E, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 31
-
 L-proline mutant enzyme K297E/E303K, at pH 7.5 and 22°C Escherichia coli
6.1.1.15 71
-
 L-proline wild type enzyme, at pH 7.5 and 22°C Escherichia coli