EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.15 | expressed in Escherichia coli SG13009 (pREP4) cells | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.15 | D198A | the overall aminoacylation activity of the mutant is reduced 5.5fold | Escherichia coli |
6.1.1.15 | E218A/N305A | inactive | Escherichia coli |
6.1.1.15 | E234A | the overall aminoacylation activity of the mutant is reduced 2fold | Escherichia coli |
6.1.1.15 | F415A | the aminoacylation activity of the mutant is nearly abolished with rates 70fold slower than the wild type | Escherichia coli |
6.1.1.15 | G412A | the overall aminoacylation activity of the mutant is reduced 7fold | Escherichia coli |
6.1.1.15 | H302A | the overall aminoacylation activity of the mutant is reduced 2fold | Escherichia coli |
6.1.1.15 | H302A/G412A | the overall aminoacylation activity of the mutant is reduced 5.5fold | Escherichia coli |
6.1.1.15 | N305A | the aminoacylation activity of the mutant is nearly abolished with rates 70fold slower than the wild type | Escherichia coli |
6.1.1.15 | N305A/G412A | inactive | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.18 | - |
L-proline | wild type enzyme, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.22 | - |
L-proline | mutant enzyme H302A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.3 | - |
L-proline | mutant enzyme G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.33 | - |
L-proline | mutant enzyme D198A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.45 | - |
L-proline | mutant enzyme N305A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.62 | - |
L-proline | mutant enzyme H302A/G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.76 | - |
L-proline | mutant enzyme F415A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 1.03 | - |
L-proline | mutant enzyme E234A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 3.4 | - |
L-proline | mutant enzyme E218A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-proline + tRNAPro | Escherichia coli | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.15 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.15 | Talon cobalt affinity resin column chromatography, and gel filtration | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.15 | Prolyl-tRNA synthetase | - |
Escherichia coli |
6.1.1.15 | ProRS | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.131 | - |
L-proline | mutant enzyme F415A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 0.61 | - |
L-proline | mutant enzyme N305A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 4.4 | - |
L-proline | mutant enzyme E218A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 6.7 | - |
L-proline | mutant enzyme E234A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 6.98 | - |
L-proline | mutant enzyme D198A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 7.3 | - |
L-proline | mutant enzyme H302A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 10.7 | - |
L-proline | mutant enzyme H302A/G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 12.6 | - |
L-proline | wild type enzyme, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 12.8 | - |
L-proline | mutant enzyme G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.15 | ATP | - |
Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.17 | - |
L-proline | mutant enzyme F415A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 1.3 | - |
L-proline | mutant enzyme E218A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 1.4 | - |
L-proline | mutant enzyme N305A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 6.5 | - |
L-proline | mutant enzyme E234A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 17 | - |
L-proline | mutant enzyme H302A/G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 21 | - |
L-proline | mutant enzyme D198A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 33 | - |
L-proline | mutant enzyme H302A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 43 | - |
L-proline | mutant enzyme G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli | |
6.1.1.15 | 71 | - |
L-proline | wild type enzyme, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C | Escherichia coli |