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Literature summary extracted from
- Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates (2012), Biochemistry, 51, 7064-7077.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.16 | expression of wild-type and mutant enzymes in yeast microsomes | Homo sapiens |
| 1.14.14.19 | expression of wild-type and mutant enzymes in yeast microsomes, expression of modified CYP17A1 in Escherichia coli strain JM109 | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.16 | V359A | site-directed mutagenesis, the mutant shows altered product formation compared to the wild-type enzyme | Homo sapiens |
| 1.14.14.19 | A105L | site-directed mutagenesis, the mutant shows low 21-hydroxylation activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.16 | additional information | - |
additional information | calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes with substrates 21-[2H]-progesterone, 21,21,21-[2H3]-progesterone, and 21,21,21-[2H3]-17-hydroxyprogesterone, overview | Homo sapiens | |
| 1.14.14.19 | additional information | - |
additional information | calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes, overview | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.16 | microsome | - |
Homo sapiens | - |
- |
| 1.14.14.19 | microsome | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.16 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| 1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.19 | pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.19 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxyprogesterone + 16alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.16 | Homo sapiens | - |
- |
- |
| 1.14.14.19 | Homo sapiens | P05093 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.16 | recombinant wild-type and mutant enzymes from yeast microsomes | Homo sapiens |
| 1.14.14.19 | recombinant wild-type and mutant enzymes from yeast mcirosomes, recombinant modified CYP17A1 from Escherichia coli strain JM109 to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.16 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.16 | additional information | human CYP21A2 also has progesterone 16alpha-hydroxylase activity. Product analysis by LC-MS/MS | Homo sapiens | ? | - |
? | |
| 1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | mutant V359A produces an additional metabolite, 16alpha-hydroxyprogesterone of progesterone, very low activity with the wild-type enzyme | Homo sapiens | 16alpha-hydroxy-progesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product identification by TLC | ? | |
| 1.14.14.19 | additional information | human CYP17A1 also has progesterone 21-hydroxylase activity, EC 1.14.99.10. Product analysis by LC-MS/MS | Homo sapiens | ? | - |
? | |
| 1.14.14.19 | pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.19 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxyprogesterone + 16alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.16 | P450c21 | - |
Homo sapiens |
| 1.14.14.19 | 17-hydroxylase/17,20-lyase | - |
Homo sapiens |
| 1.14.14.19 | CYP17A1 | - |
Homo sapiens |
| 1.14.14.19 | P450c17 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.16 | 37 | - |
assay at | Homo sapiens |
| 1.14.14.19 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.16 | 7.4 | - |
assay at | Homo sapiens |
| 1.14.14.19 | 7.4 | - |
assay at | Homo sapiens |
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