EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.149 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.149 | 0.012 | - |
crotonobetainyl-CoA | at pH 7.5 and 37°C | Escherichia coli | |
4.2.1.149 | 0.038 | - |
crotonyl-CoA | at pH 7.5 and 37°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.149 | 27000 | - |
x * 27000, SDS-PAGE | Escherichia coli |
4.2.1.149 | 87000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.149 | crotonobetainyl-CoA + H2O | Escherichia coli | - |
L-carnitinyl-CoA | - |
r | |
4.2.1.149 | crotonyl-CoA + H2O | Escherichia coli | - |
? | - |
? | |
4.2.1.149 | L-carnitinyl-CoA | Escherichia coli | - |
crotonobetainyl-CoA + H2O | - |
r | |
4.2.1.149 | additional information | Escherichia coli | does not use crotonobetaine as substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.21 | Escherichia coli | P31572 | - |
- |
4.2.1.149 | Escherichia coli | P31551 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.149 | DEAE-Sepharose column chromatography and Resource Q column chromatography | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.3.21 | 187.8 | - |
pH 7.5, 37°C | Escherichia coli |
4.2.1.149 | 60.3 | - |
cell-free extract, at pH 7.5 and 37°C | Escherichia coli |
4.2.1.149 | 699.9 | - |
after 11.6fold purification, at pH 7.5 and 37°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.21 | crotonobetainyl-CoA + L-carnitine | - |
Escherichia coli | crotonobetaine + L-carnitinyl-CoA | - |
? | |
2.8.3.21 | gamma-butyrobetainyl-CoA + L-carnitine | - |
Escherichia coli | gamma-butyrobetaine + L-carnitinyl-CoA | - |
? | |
2.8.3.21 | additional information | enzyme exhibits high cosubstrate specificity to CoA derivatives of trimethylammonium compounds. Hydration of crotonobetaine to L-carnitine proceeds at the CoA level in two steps: the CaiD catalyzed hydration of crotonobetainyl-CoA to L-carnitinyl-CoA, followed by a CoA transfer from L-carnitinyl-CoA to crotonobetaine, catalyzed by transferase CaiB. When gamma-butyrobetainyl-CoA is used as a cosubstrate, the first reaction is the CoA transfer. The optimal ratios of CaiB and CaiD during this hydration reaction, determined to be 4:1 when crotonobetainyl-CoA is used as cosubstrate and 5:1 when gamma-butyrobetainyl-CoA is used as cosubstrate, are different from that found for in vivo conditions, i.e. 1:3 | Escherichia coli | ? | - |
? | |
4.2.1.149 | crotonobetainyl-CoA + H2O | - |
Escherichia coli | L-carnitinyl-CoA | - |
r | |
4.2.1.149 | crotonyl-CoA + H2O | - |
Escherichia coli | ? | - |
? | |
4.2.1.149 | L-carnitinyl-CoA | - |
Escherichia coli | crotonobetainyl-CoA + H2O | - |
r | |
4.2.1.149 | additional information | does not use crotonobetaine as substrate | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.149 | ? | x * 27000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.3.21 | CaiB | - |
Escherichia coli |
4.2.1.149 | CaiD | - |
Escherichia coli |