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Literature summary extracted from

  • Li, S.F.; Song, L.Y.; Zhang, G.J.; Yin, W.B.; Chen, Y.H.; Wang, R.R.; Hu, Z.M.
    Newly identified essential amino acid residues affecting DELTA8-sphingolipid desaturase activity revealed by site-directed mutagenesis (2011), Biochem. Biophys. Res. Commun., 416, 165-171.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.18 mutant enzymes are expressed in Saccharomyces cerevisae strain INVScI Brassica rapa subsp. oleifera
1.14.19.29 expressed in Saccharomyces cerevisiae strain INVScI Brassica rapa

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.4 D208R inactive Brassica rapa
1.14.19.4 D210R inactive Brassica rapa
1.14.19.4 F59R the mutant shows markedly decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 G28R the mutant shows decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 G368R inactive Brassica rapa
1.14.19.4 G52R the mutant shows wild type activity Brassica rapa
1.14.19.4 H63R inactive Brassica rapa
1.14.19.4 L369A the mutant shows markedly decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 L71R the mutant shows wild type activity Brassica rapa
1.14.19.4 N203R inactive Brassica rapa
1.14.19.4 Q372H the mutant shows markedly decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 T56R the mutant shows decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 W190A the mutant shows markedly decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 W345A the mutant shows markedly decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.4 Y31A the mutant shows decreased activity compared to the wild type enzyme Brassica rapa
1.14.19.18 D208R inactive Brassica rapa subsp. oleifera
1.14.19.18 D210R inactive Brassica rapa subsp. oleifera
1.14.19.18 F59R the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 G28R the mutant shows slightly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 G368R inactive Brassica rapa subsp. oleifera
1.14.19.18 G52R the mutant shows wild type activity Brassica rapa subsp. oleifera
1.14.19.18 H63R inactive Brassica rapa subsp. oleifera
1.14.19.18 L369A the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 L71R the mutant shows wild type activity Brassica rapa subsp. oleifera
1.14.19.18 N203R inactive Brassica rapa subsp. oleifera
1.14.19.18 Q372H the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 T56R the mutant shows slightly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 W190A the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 W345A the mutant shows markedly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.18 Y31A the mutant shows slightly decreased conversion rate compared to the wild type enzyme Brassica rapa subsp. oleifera
1.14.19.29 D208R inactive Brassica rapa
1.14.19.29 D210R inactive Brassica rapa
1.14.19.29 F59R the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 G28R the mutant shows reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 G368R inactive Brassica rapa
1.14.19.29 G52R the mutant shows wild type activity Brassica rapa
1.14.19.29 H63R inactive Brassica rapa
1.14.19.29 L369A the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 L71R the mutant shows wild type activity Brassica rapa
1.14.19.29 N203R inactive Brassica rapa
1.14.19.29 Q372H the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 T56R the mutant shows reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 W190A the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 W345A the mutant shows severely reduced activity compared to the wild type enzyme Brassica rapa
1.14.19.29 Y31A the mutant shows reduced activity compared to the wild type enzyme Brassica rapa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.4 membrane
-
Brassica rapa 16020
-
1.14.19.18 membrane
-
Brassica rapa subsp. oleifera 16020
-
1.14.19.29 endoplasmic reticulum
-
Brassica rapa 5783
-
1.14.19.29 membrane
-
Brassica rapa 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.18 (4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ Brassica rapa subsp. oleifera
-
(4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O
-
?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Brassica rapa
-
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Brassica rapa
-
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Brassica rapa
-
-
-
1.14.19.18 Brassica rapa subsp. oleifera G9I7G1
-
-
1.14.19.29 Brassica rapa G9I7G1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.18 (4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+
-
Brassica rapa subsp. oleifera (4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O
-
?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Brassica rapa (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Brassica rapa (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 D8A
-
Brassica rapa
1.14.19.4 DELTA8-sphingolipid desaturase
-
Brassica rapa
1.14.19.18 DELTA8-sphingolipid desaturase
-
Brassica rapa subsp. oleifera
1.14.19.29 D8A
-
Brassica rapa
1.14.19.29 DELTA8-sphingolipid desaturase
-
Brassica rapa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.18 cytochrome b5
-
Brassica rapa subsp. oleifera
1.14.19.29 cytochrome b5
-
Brassica rapa