EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.18 | mutant enzymes are expressed in Saccharomyces cerevisae strain INVScI | Brassica rapa subsp. oleifera |
1.14.19.29 | expressed in Saccharomyces cerevisiae strain INVScI | Brassica rapa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.19.4 | D208R | inactive | Brassica rapa |
1.14.19.4 | D210R | inactive | Brassica rapa |
1.14.19.4 | F59R | the mutant shows markedly decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | G28R | the mutant shows decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | G368R | inactive | Brassica rapa |
1.14.19.4 | G52R | the mutant shows wild type activity | Brassica rapa |
1.14.19.4 | H63R | inactive | Brassica rapa |
1.14.19.4 | L369A | the mutant shows markedly decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | L71R | the mutant shows wild type activity | Brassica rapa |
1.14.19.4 | N203R | inactive | Brassica rapa |
1.14.19.4 | Q372H | the mutant shows markedly decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | T56R | the mutant shows decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | W190A | the mutant shows markedly decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | W345A | the mutant shows markedly decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.4 | Y31A | the mutant shows decreased activity compared to the wild type enzyme | Brassica rapa |
1.14.19.18 | D208R | inactive | Brassica rapa subsp. oleifera |
1.14.19.18 | D210R | inactive | Brassica rapa subsp. oleifera |
1.14.19.18 | F59R | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | G28R | the mutant shows slightly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | G368R | inactive | Brassica rapa subsp. oleifera |
1.14.19.18 | G52R | the mutant shows wild type activity | Brassica rapa subsp. oleifera |
1.14.19.18 | H63R | inactive | Brassica rapa subsp. oleifera |
1.14.19.18 | L369A | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | L71R | the mutant shows wild type activity | Brassica rapa subsp. oleifera |
1.14.19.18 | N203R | inactive | Brassica rapa subsp. oleifera |
1.14.19.18 | Q372H | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | T56R | the mutant shows slightly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | W190A | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | W345A | the mutant shows markedly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.18 | Y31A | the mutant shows slightly decreased conversion rate compared to the wild type enzyme | Brassica rapa subsp. oleifera |
1.14.19.29 | D208R | inactive | Brassica rapa |
1.14.19.29 | D210R | inactive | Brassica rapa |
1.14.19.29 | F59R | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | G28R | the mutant shows reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | G368R | inactive | Brassica rapa |
1.14.19.29 | G52R | the mutant shows wild type activity | Brassica rapa |
1.14.19.29 | H63R | inactive | Brassica rapa |
1.14.19.29 | L369A | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | L71R | the mutant shows wild type activity | Brassica rapa |
1.14.19.29 | N203R | inactive | Brassica rapa |
1.14.19.29 | Q372H | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | T56R | the mutant shows reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | W190A | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | W345A | the mutant shows severely reduced activity compared to the wild type enzyme | Brassica rapa |
1.14.19.29 | Y31A | the mutant shows reduced activity compared to the wild type enzyme | Brassica rapa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.4 | membrane | - |
Brassica rapa | 16020 | - |
1.14.19.18 | membrane | - |
Brassica rapa subsp. oleifera | 16020 | - |
1.14.19.29 | endoplasmic reticulum | - |
Brassica rapa | 5783 | - |
1.14.19.29 | membrane | - |
Brassica rapa | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.18 | (4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ | Brassica rapa subsp. oleifera | - |
(4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O | - |
? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Brassica rapa | - |
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Brassica rapa | - |
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.4 | Brassica rapa | - |
- |
- |
1.14.19.18 | Brassica rapa subsp. oleifera | G9I7G1 | - |
- |
1.14.19.29 | Brassica rapa | G9I7G1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.18 | (4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Brassica rapa subsp. oleifera | (4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O | - |
? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Brassica rapa | (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Brassica rapa | (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.4 | D8A | - |
Brassica rapa |
1.14.19.4 | DELTA8-sphingolipid desaturase | - |
Brassica rapa |
1.14.19.18 | DELTA8-sphingolipid desaturase | - |
Brassica rapa subsp. oleifera |
1.14.19.29 | D8A | - |
Brassica rapa |
1.14.19.29 | DELTA8-sphingolipid desaturase | - |
Brassica rapa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.18 | cytochrome b5 | - |
Brassica rapa subsp. oleifera | |
1.14.19.29 | cytochrome b5 | - |
Brassica rapa |