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Literature summary extracted from
- Variable composition of heme oxygenases with different regiospecificities in Pseudomonas species (2012), Arch. Microbiol., 194, 597-606.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.58 | gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3) | Pseudomonas mendocina |
| 1.14.99.58 | gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
| 1.14.99.58 | gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3) | Pseudomonas fluorescens |
| 1.14.99.58 | gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of N-terminally His6-tagged BphO in Escherichia coli strain BL21(DE3) | Pseudomonas syringae pv. tomato |
| 1.14.99.58 | gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
| 1.14.99.58 | gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) | Pseudomonas fluorescens |
| 1.14.99.58 | gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) | Pseudomonas mendocina |
| 1.14.99.58 | gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) | Pseudomonas syringae pv. tomato |
| 1.14.99.58 | genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
| 1.14.99.58 | genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.99.58 | additional information | - |
additional information | Michaelis-Menten kinetics for PigA, overview | Pseudomonas aeruginosa | |
| 1.14.99.58 | additional information | - |
additional information | Michaelis-Menten kinetics for PigA, overview | Pseudomonas fluorescens | |
| 1.14.99.58 | additional information | - |
additional information | Michaelis-Menten kinetics for PigA, overview | Pseudomonas putida | |
| 1.14.99.58 | 0.0242 | - |
heme | PigA, pH and temperature not specified in the publication | Pseudomonas fluorescens |  |
| 1.14.99.58 | 0.0269 | - |
heme | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 1.14.99.58 | 0.0269 | - |
heme | pH and temperature not specified in the publication | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas syringae pv. tomato | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas aeruginosa | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas fluorescens | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas mendocina | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas putida | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas aeruginosa | natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas fluorescens Pf-5 | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas syringae pv. tomato DC3000 | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas aeruginosa KT 2240 | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas putida KT 2240 | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | Pseudomonas mendocina YMP | - |
biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas aeruginosa | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas putida | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas syringae pv. tomato | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas fluorescens | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas mendocina | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas fluorescens Pf-5 | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas syringae pv. tomato DC3000 | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas aeruginosa KT 2240 | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas putida KT 2240 | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | ? | - |
? | |
| 1.14.99.58 | additional information | Pseudomonas mendocina YMP | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.58 | Pseudomonas aeruginosa | O69002 | PigA; gene pigA | - |
| 1.14.99.58 | Pseudomonas aeruginosa | Q88P48 | gene hemO; two genes pigA encoding PigA homologues | - |
| 1.14.99.58 | Pseudomonas aeruginosa | Q9HWR4 | BphO; gene bphO | - |
| 1.14.99.58 | Pseudomonas aeruginosa KT 2240 | Q88P48 | gene hemO; two genes pigA encoding PigA homologues | - |
| 1.14.99.58 | Pseudomonas fluorescens | Q4K657 | BphO; gene bphO | - |
| 1.14.99.58 | Pseudomonas fluorescens | Q4K7S1 | PigA; gene pigA | - |
| 1.14.99.58 | Pseudomonas fluorescens Pf-5 | Q4K657 | BphO; gene bphO | - |
| 1.14.99.58 | Pseudomonas fluorescens Pf-5 | Q4K7S1 | PigA; gene pigA | - |
| 1.14.99.58 | Pseudomonas mendocina | A4Y0Y8 | - |
- |
| 1.14.99.58 | Pseudomonas mendocina | A4Y0Y8 | PigA; gene pigA | - |
| 1.14.99.58 | Pseudomonas mendocina YMP | A4Y0Y8 | - |
- |
| 1.14.99.58 | Pseudomonas mendocina YMP | A4Y0Y8 | PigA; gene pigA | - |
| 1.14.99.58 | Pseudomonas putida | Q88JR7 | two genes pigA encoding PigA homologues | - |
| 1.14.99.58 | Pseudomonas putida KT 2240 | Q88JR7 | two genes pigA encoding PigA homologues | - |
| 1.14.99.58 | Pseudomonas syringae pv. tomato | Q885D4 | BphO; pv. tomato, gene bphO | - |
| 1.14.99.58 | Pseudomonas syringae pv. tomato | Q887K9 | PigA; pv. tomato, gene pigA | - |
| 1.14.99.58 | Pseudomonas syringae pv. tomato DC3000 | Q885D4 | BphO; pv. tomato, gene bphO | - |
| 1.14.99.58 | Pseudomonas syringae pv. tomato DC3000 | Q887K9 | PigA; pv. tomato, gene pigA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.58 | recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas mendocina |
| 1.14.99.58 | recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas aeruginosa |
| 1.14.99.58 | recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas fluorescens |
| 1.14.99.58 | recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by affinity chromatography | Pseudomonas syringae pv. tomato |
| 1.14.99.58 | recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas aeruginosa |
| 1.14.99.58 | recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas fluorescens |
| 1.14.99.58 | recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas mendocina |
| 1.14.99.58 | recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Pseudomonas putida |
| 1.14.99.58 | recombinant N-terminally His6-tagged BphO from Escherichia coli strain BL21(DE3) by affinity chromatography | Pseudomonas syringae pv. tomato |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas syringae pv. tomato | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas aeruginosa | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas fluorescens | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas mendocina | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas putida | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase | Pseudomonas aeruginosa | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas syringae pv. tomato | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas aeruginosa | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas fluorescens | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas mendocina | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas putida | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ferredoxin from spinach, and spinach ferredoxin-NADP+-oxidoreductase | Pseudomonas aeruginosa | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas fluorescens Pf-5 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas fluorescens Pf-5 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas syringae pv. tomato DC3000 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas syringae pv. tomato DC3000 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas aeruginosa KT 2240 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas aeruginosa KT 2240 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas putida KT 2240 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas putida KT 2240 | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | - |
Pseudomonas mendocina YMP | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | heme + 3 AH2 + 3 O2 | with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination | Pseudomonas mendocina YMP | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
| 1.14.99.58 | additional information | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | Pseudomonas aeruginosa | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | Pseudomonas putida | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas syringae pv. tomato | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas aeruginosa | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas fluorescens | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas mendocina | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas fluorescens Pf-5 | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas syringae pv. tomato DC3000 | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | Pseudomonas aeruginosa KT 2240 | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha | Pseudomonas putida KT 2240 | ? | - |
? | |
| 1.14.99.58 | additional information | the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta | Pseudomonas mendocina YMP | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.58 | BphO | - |
Pseudomonas syringae pv. tomato |
| 1.14.99.58 | BphO | - |
Pseudomonas mendocina |
| 1.14.99.58 | BphO | - |
Pseudomonas aeruginosa |
| 1.14.99.58 | BphO | - |
Pseudomonas fluorescens |
| 1.14.99.58 | PigA | - |
Pseudomonas aeruginosa |
| 1.14.99.58 | PigA | - |
Pseudomonas fluorescens |
| 1.14.99.58 | PigA | - |
Pseudomonas mendocina |
| 1.14.99.58 | PigA | - |
Pseudomonas syringae pv. tomato |
| 1.14.99.58 | PigA | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.58 | 45 | - |
above, PigA | Pseudomonas aeruginosa |
| 1.14.99.58 | 45 | - |
above, PigA | Pseudomonas fluorescens |
| 1.14.99.58 | 45 | - |
above, PigA | Pseudomonas mendocina |
| 1.14.99.58 | 45 | - |
above, PigA | Pseudomonas syringae pv. tomato |
| 1.14.99.58 | 45 | - |
above, PigA | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.58 | 6 | - |
PigA | Pseudomonas fluorescens |
| 1.14.99.58 | 6.75 | - |
PigA | Pseudomonas aeruginosa |
| 1.14.99.58 | 6.75 | - |
PigA | Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.58 | 6 | 9 | high activity of PigA within this range | Pseudomonas aeruginosa |
| 1.14.99.58 | 6 | 9 | high activity of PigA within this range | Pseudomonas fluorescens |
| 1.14.99.58 | 6 | 9 | high activity of PigA within this range | Pseudomonas mendocina |
| 1.14.99.58 | 6 | 9 | high activity of PigA within this range | Pseudomonas syringae pv. tomato |
| 1.14.99.58 | 6 | 9 | high activity of PigA within this range | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.58 | ferredoxin-NADP+-oxidoreductase | - |
Pseudomonas syringae pv. tomato | |
| 1.14.99.58 | ferredoxin-NADP+-oxidoreductase | - |
Pseudomonas aeruginosa | |
| 1.14.99.58 | ferredoxin-NADP+-oxidoreductase | - |
Pseudomonas fluorescens | |
| 1.14.99.58 | ferredoxin-NADP+-oxidoreductase | - |
Pseudomonas mendocina | |
| 1.14.99.58 | ferredoxin-NADP+-oxidoreductase | - |
Pseudomonas putida | |
| 1.14.99.58 | additional information | with PigA, there is no difference in product formation whether ferredoxin-NADP+-oxidoreductase or ferredoxin, FNR and ascorbate are used as electron donor | Pseudomonas aeruginosa |
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