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Literature summary extracted from

  • Shima, S.; Herault, D.A.; Berkessel, A.; Thauer, R.K.
    Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri (1998), Arch. Microbiol., 170, 469-472.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.101 2,3-diphosphoglycerate the 1 M 2,3-diphosphoglycerate the enzyme is highly active Methanopyrus kandleri
3.5.4.27 2,3-bisphosphoglycerate the potassium salts of 2,3-bisphosphoglycerate stimulates cyclohydrolase activity Methanopyrus kandleri
3.5.4.27 2,3-diphosphoglycerate the activity increases with increasing 2,3-diphosphoglycerate concentrations from 30 U/mg up to a specific activity of 7,500 U/mg. The maximal activity is reached at a 2,3-diphosphoglycerate concentration of 0.75 M. At higher concentrations, the activity decreases Methanopyrus kandleri
3.5.4.27 phosphate the potassium salts of phosphateglycerate stimulates cyclohydrolase activity Methanopyrus kandleri

General Stability

EC Number General Stability Organism
2.3.1.101 at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri
3.5.4.27 at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methanopyrus kandleri
-
-
-
3.5.4.27 Methanopyrus kandleri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.101 stable under oxic conditions Methanopyrus kandleri
3.5.4.27 stable under oxic conditions Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.101 formylmethanofuran:tetrahydromethanopterin formyltransferase
-
 Methanopyrus kandleri
3.5.4.27 N5,N10-methenyltetrahydromethanopterin cyclohydrolase
-
 Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.101 65
-
 assay at Methanopyrus kandleri
3.5.4.27 65
-
 assay at Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.101 additional information
-
 at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri
2.3.1.101 90
-
 in 0.5 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C. Methanopyrus kandleri
3.5.4.27 additional information
-
 at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri
3.5.4.27 90
-
 in 0.75 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C Methanopyrus kandleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.101 8
-
 assay at Methanopyrus kandleri
3.5.4.27 8
-
 assay at Methanopyrus kandleri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.27 8
-
 in 0.75 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C Methanopyrus kandleri