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Literature summary extracted from

  • Kowatz, T.; Babino, D.; Kiser, P.; Palczewski, K.; von Lintig, J.
    Characterization of human beta,beta-carotene-15,15'-monooxygenase (BCMO1) as a soluble monomeric enzyme (2013), Arch. Biochem. Biophys., 539, 214-222.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.63 gene BCMO1, expression of enzyme with C-terminal C-terminal TEV-, His6- and 1D4-tags in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.63 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate at 0.245% Homo sapiens
1.13.11.63 hexaethylene glycol monooctyl ether at 0.195-0.78% Homo sapiens
1.13.11.63 additional information addition of detergents fails to increase BCMO1 enzymatic activity, but short chain aliphatic detergents such as C8E4 and C8E6 decrease enzymatic activity probably by interacting with the substrate binding site Homo sapiens
1.13.11.63 n-octyl-beta-D-thioglucopyranoside at 0.56% Homo sapiens
1.13.11.63 tetraethylene glycol monooctyl ether at 0.125-0.5% Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.63 additional information
-
 additional information Michaelis-Menten kinetics, overview Homo sapiens
1.13.11.63 0.0137 0.0148 beta-carotene recombinant enzyme, pH 7.5, temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.63 cytosol
-
 Mus musculus 5829
-
1.13.11.63 cytosol
-
 Homo sapiens 5829
-
1.13.11.63 additional information determination of BCMO1 subcellular localization in mouse liver, overview Mus musculus
-
-
1.13.11.63 soluble BCMO1 is soluble and its enzymatic activity is not dependent on a membrane-like environment Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.63 Fe2+ the enzyme is a non-heme iron oxygenase Mus musculus
1.13.11.63 Fe2+ the enzyme is a non-heme iron oxygenase Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.63 60000
-
 recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.63 beta-carotene + O2 Mus musculus
-
 2 all-trans-retinal
-
 ?
1.13.11.63 beta-carotene + O2 Homo sapiens
-
 2 all-trans-retinal
-
 ?
1.13.11.63 beta-carotene + O2 Mus musculus C57/BL6 / 129 Sv
-
 2 all-trans-retinal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Homo sapiens Q9HAY6 gene Bcmo1
-
1.13.11.63 Mus musculus Q9JJS6 gene Bcmo1
-
1.13.11.63 Mus musculus C57/BL6 / 129 Sv Q9JJS6 gene Bcmo1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.63 native enzyme partially from Stellate cell cytosol Mus musculus
1.13.11.63 recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags from Spodoptera frugiperda Sf9 cells by Co2+ affinity chromatography and gel filtration in absence of detergent Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.63 liver
-
 Mus musculus
-
1.13.11.63 stellate cell high enzyme expression Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 beta-carotene + O2
-
 Mus musculus 2 all-trans-retinal
-
 ?
1.13.11.63 beta-carotene + O2
-
 Homo sapiens 2 all-trans-retinal
-
 ?
1.13.11.63 beta-carotene + O2
-
 Mus musculus C57/BL6 / 129 Sv 2 all-trans-retinal
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.63 monomer
-
 Mus musculus
1.13.11.63 monomer 1 x 65200, recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 Bcmo1
-
 Mus musculus
1.13.11.63 Bcmo1
-
 Homo sapiens
1.13.11.63 beta,beta-carotene-15,15'-monooxygenase
-
 Mus musculus
1.13.11.63 beta,beta-carotene-15,15'-monooxygenase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.63 7.5 8.5 beta-carotene recombinant enzyme, pH 7.5, temperature not specified in the publication Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.63 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.63 additional information recombinant BCMO1 does not require cofactors Homo sapiens