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Literature summary extracted from

  • Terrell, C.R.; Burks, E.A.; Whitman, C.P.; Hoffman, D.W.
    Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1 (2013), Arch. Biochem. Biophys., 537, 113-124.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.2.6 expressed in Escherichia coli BL21(DE3) cells Methylibium petroleiphilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.2.6 hanging drop vapor diffusion method, using 200 mM tri-ammonium citrate and 10% (w/v)polyethylene glycol (PEG) 3350 Methylibium petroleiphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.2.6 0.06
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 0.105
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 0.14
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 0.211
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate Methylibium petroleiphilum
-
 (3E)-2-oxohex-3-enedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.2.6 Methylibium petroleiphilum A2SL37
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.2.6 DEAE-Sepharose column chromatography and SP-Sepharose column chromatography Methylibium petroleiphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Methylibium petroleiphilum (3E)-2-oxohex-3-enedioate
-
 ?
5.3.2.6 2-hydroxymuconate
-
 Methylibium petroleiphilum 2-oxo-3-hexenedioate
-
 ?
5.3.2.6 5-(methyl)-2-hydroxymuconate
-
 Methylibium petroleiphilum 5-(methyl)-2-oxo-3-hexenedioate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.2.6 4-OT
-
 Methylibium petroleiphilum
5.3.2.6 4-oxalocrotonate tautomerase I isoform Methylibium petroleiphilum
5.3.2.6 4-oxalocrotonate tautomerase II isoform Methylibium petroleiphilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.2.6 3.4
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 18.5
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 107
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 4600
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.2.6 16
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 310
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 1000
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
5.3.2.6 33000
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum