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Literature summary extracted from

  • Franco, T.M.; Rostirolla, D.C.; Ducati, R.G.; Lorenzini, D.M.; Basso, L.A.; Santos, D.S.
    Biochemical characterization of recombinant guaA-encoded guanosine monophosphate synthetase (EC 6.3.5.2) from Mycobacterium tuberculosis H37Rv strain (2012), Arch. Biochem. Biophys., 517, 1-11.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.2 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.2 ATP substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 0.027
-
 ATP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis
6.3.5.2 1.24
-
 L-glutamine at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.2 Mg2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.2 56000
-
 2 * 56000, SDS-PAGE Mycobacterium tuberculosis
6.3.5.2 137000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.2 ATP + XMP + L-glutamine + H2O Mycobacterium tuberculosis
-
 AMP + diphosphate + GMP + L-glutamate
-
 ?
6.3.5.2 ATP + XMP + L-glutamine + H2O Mycobacterium tuberculosis H37Rv
-
 AMP + diphosphate + GMP + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Mycobacterium tuberculosis
-
-
-
6.3.5.2 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.2 Q-Sepharose column chromatography, butyl Sepharose column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
6.3.5.2 -80°C, in 50 mM Tris pH 7.5, at least 3 months, no loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + XMP + L-glutamine + H2O
-
 Mycobacterium tuberculosis AMP + diphosphate + GMP + L-glutamate
-
 ?
6.3.5.2 ATP + XMP + L-glutamine + H2O
-
 Mycobacterium tuberculosis H37Rv AMP + diphosphate + GMP + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.2 homodimer 2 * 56000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.3.5.2 GMP synthetase
-
 Mycobacterium tuberculosis
6.3.5.2 GMPS
-
 Mycobacterium tuberculosis
6.3.5.2 GUA
-
 Mycobacterium tuberculosis
6.3.5.2 guanosine monophosphate synthetase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.2 2.3
-
 XMP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis
6.3.5.2 2.39
-
 ATP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis
6.3.5.2 3.05
-
 L-glutamine at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.2 7.5
-
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.2 ATP
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.5.2 21
-
 ATP at 40°C in 50 mM Tris pH 7.5 Mycobacterium tuberculosis