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Literature summary extracted from

  • Kim, H.S.; Kwak, G.H.; Lee, K.; Jo, C.H.; Hwang, K.Y.; Kim, H.Y.
    Structural and biochemical analysis of a type II free methionine-R-sulfoxide reductase from Thermoplasma acidophilum (2014), Arch. Biochem. Biophys., 560, 10-9.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.14
-
 Thermoplasma acidophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.14 hanging-drop vapor diffusion method at 20°C. Crystal structure is determined in native form and in a complex with the substrate Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.14 C15S mutant exhibits apparent activity. The kcat and Km values of the mutant enzyme are similar to those of wild type, showing that Cys15 is dispensable for catalysis Thermoplasma acidophilum
1.8.4.14 C60S mutant exhibits apparent activity. The kcat value is dramatically reduced in the C60S mutant (80fold lower), while the Km value does not significantly change compared to wild type Thermoplasma acidophilum
1.8.4.14 C84S no detectable activity. Cells expressing C84S do not grow in the Met-O medium at all Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.14 0.33
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
1.8.4.14 0.4
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum
1.8.4.14 0.47
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.14 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.14
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.14 L-methionine (R)-S-oxide + thioredoxin the enzyme only reduces the free form of methionine-R-sulfoxide Thermoplasma acidophilum L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.14 methionine-R-sulfoxide reductase
-
 Thermoplasma acidophilum
1.8.4.14 TafRMsr
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.14 0.0033
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
1.8.4.14 0.268
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum
1.8.4.14 0.29
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.14 0.01
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C60S Thermoplasma acidophilum
1.8.4.14 0.62
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, mutant enzyme C15S Thermoplasma acidophilum
1.8.4.14 0.67
-
 L-methionine (R)-S-oxide pH 7.5, 25°C, wild-type enzyme Thermoplasma acidophilum