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Literature summary extracted from

  • Dudek, H.M.; Fink, M.J.; Shivange, A.V.; Dennig, A.; Mihovilovic, M.D.; Schwaneberg, U.; Fraaije, M.W.
    Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis (2013), Appl. Microbiol. Biotechnol., 98, 4009-4020.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.92 expressed in Escherichia coli BL21(DE3) cells Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.92 P253F/G254A/R258M/L443F the mutant shows the same thermostability as the wild type enzyme while it displays an extended substrate spectrum Thermobifida fusca
1.14.13.92 W501A the mutant shows reduced activity compared to the wild type enzyme Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.92 2-Octanone substrate inhibition Thermobifida fusca
1.14.13.92 phenylacetone substrate inhibition Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.92 0.04
-
phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.08
-
phenylacetone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.25
-
2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.8
-
2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 2
-
2-Octanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 4
-
2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 1000
-
Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 1200
-
Cyclopentanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.92 phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
benzyl acetate + NADP+ + H2O
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.13.92 additional information the initial activity of wild type enzyme is not affected by 10% (v/v) 1,4-dioxane Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 (2R,3S)-3-methyl-2-pentylcyclopentanone + NADPH + H+ + O2 less than 5% conversion Thermobifida fusca ?
-
?
1.14.13.92 2-benzylcyclopentanone + NADPH + H+ + O2 about 10% conversion Thermobifida fusca ?
-
?
1.14.13.92 2-nonanone + NADPH + H+ + O2 less than 40% conversion Thermobifida fusca ?
-
?
1.14.13.92 2-octanone + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?
1.14.13.92 2-phenylcyclohexanone + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?
1.14.13.92 3-(4-chlorophenyl)cyclobutanone + NADPH + H+ + O2 about 40% conversion Thermobifida fusca ?
-
?
1.14.13.92 3-benzylcyclobutanone + NADPH + H+ + O2 about 45% conversion Thermobifida fusca ?
-
?
1.14.13.92 3-octanone + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?
1.14.13.92 3-phenylcyclobutanone + NADPH + H+ + O2 about 70% conversion Thermobifida fusca ?
-
?
1.14.13.92 bicyclo[2.2.1]heptan-2-one + NADPH + H+ + O2 about 5% conversion Thermobifida fusca ?
-
?
1.14.13.92 bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?
1.14.13.92 cyclohexanone + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?
1.14.13.92 cyclopentanone + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
Thermobifida fusca benzyl acetate + NADP+ + H2O
-
?
1.14.13.92 thioanisole + NADPH + H+ + O2
-
Thermobifida fusca ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 Baeyer-Villiger monooxygenase
-
Thermobifida fusca
1.14.13.92 PAMO
-
Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.92 60
-
the wild type enzyme has a melting temperature of 60°C Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.92 0.07
-
2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.3
-
2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.9
-
Cyclopentanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 1
-
2-Octanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 1.4
-
phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 1.6
-
Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 2.3
-
2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 3
-
phenylacetone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 NADPH
-
Thermobifida fusca

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.92 0.008
-
2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.37
-
2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.48
-
2-Octanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 0.8
-
Cyclopentanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 1.6
-
Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 9.2
-
2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 35
-
phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.14.13.92 37.5
-
phenylacetone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca