EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.92 | expressed in Escherichia coli BL21(DE3) cells | Thermobifida fusca |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.92 | P253F/G254A/R258M/L443F | the mutant shows the same thermostability as the wild type enzyme while it displays an extended substrate spectrum | Thermobifida fusca |
1.14.13.92 | W501A | the mutant shows reduced activity compared to the wild type enzyme | Thermobifida fusca |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.92 | 2-Octanone | substrate inhibition | Thermobifida fusca | |
1.14.13.92 | phenylacetone | substrate inhibition | Thermobifida fusca |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.92 | 0.04 | - |
phenylacetone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.08 | - |
phenylacetone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.25 | - |
2-Octanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.8 | - |
2-phenylcyclohexanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 2 | - |
2-Octanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 4 | - |
2-phenylcyclohexanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 1000 | - |
Cyclopentanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 1200 | - |
Cyclopentanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.92 | phenylacetone + NADPH + H+ + O2 | Thermobifida fusca | - |
benzyl acetate + NADP+ + H2O | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.14.13.92 | additional information | the initial activity of wild type enzyme is not affected by 10% (v/v) 1,4-dioxane | Thermobifida fusca |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.92 | Thermobifida fusca | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.92 | (2R,3S)-3-methyl-2-pentylcyclopentanone + NADPH + H+ + O2 | less than 5% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 2-benzylcyclopentanone + NADPH + H+ + O2 | about 10% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 2-nonanone + NADPH + H+ + O2 | less than 40% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 2-octanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 2-phenylcyclohexanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 3-(4-chlorophenyl)cyclobutanone + NADPH + H+ + O2 | about 40% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 3-benzylcyclobutanone + NADPH + H+ + O2 | about 45% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 3-octanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
1.14.13.92 | 3-phenylcyclobutanone + NADPH + H+ + O2 | about 70% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | bicyclo[2.2.1]heptan-2-one + NADPH + H+ + O2 | about 5% conversion | Thermobifida fusca | ? | - |
? | |
1.14.13.92 | bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
1.14.13.92 | cyclohexanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
1.14.13.92 | cyclopentanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
1.14.13.92 | phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? | |
1.14.13.92 | thioanisole + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.92 | Baeyer-Villiger monooxygenase | - |
Thermobifida fusca |
1.14.13.92 | PAMO | - |
Thermobifida fusca |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.92 | 60 | - |
the wild type enzyme has a melting temperature of 60°C | Thermobifida fusca |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.92 | 0.07 | - |
2-phenylcyclohexanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.3 | - |
2-phenylcyclohexanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.9 | - |
Cyclopentanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 1 | - |
2-Octanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 1.4 | - |
phenylacetone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 1.6 | - |
Cyclopentanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 2.3 | - |
2-Octanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 3 | - |
phenylacetone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.92 | NADPH | - |
Thermobifida fusca |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.92 | 0.008 | - |
2-phenylcyclohexanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.37 | - |
2-phenylcyclohexanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.48 | - |
2-Octanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 0.8 | - |
Cyclopentanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 1.6 | - |
Cyclopentanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 9.2 | - |
2-Octanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 35 | - |
phenylacetone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.14.13.92 | 37.5 | - |
phenylacetone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca |