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Literature summary extracted from

  • Hotta, Y.; Ezaki, S.; Atomi, H.; Imanaka, T.
    Extremely stable and versatile carboxylesterase from a hyperthermophilic archaeon (2002), Appl. Environ. Microbiol., 68, 3925-3931.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1
-
 Pyrobaculum calidifontis

General Stability

EC Number General Stability Organism
3.1.1.1 0.1% Triton X-100 exhibits a significant stabilizing effect without leading to a change in activity levels Pyrobaculum calidifontis
3.1.1.1 unstable at low concentrations, 10 - 100 nM. The half-life of the enzyme at a concentration of 60 nM is 5.2 h in 20 mM Tris-HCl (pH 8.0) at 4°C Pyrobaculum calidifontis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 diethyldicarbonate 2.0 mM, 98% inhibition Pyrobaculum calidifontis
3.1.1.1 PMSF 0.025, 94% inhibition Pyrobaculum calidifontis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.044
-
 4-nitrophenyl hexanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 0.051
-
 4-nitrophenyl octanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 0.071
-
 4-nitrophenyl pentanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 0.164
-
 4-nitrophenyl butanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 0.348
-
 4-nitrophenyl propanoate pH 7.1, 70°C Pyrobaculum calidifontis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 34000
-
 3 * 34000, SDS-PAGE Pyrobaculum calidifontis
3.1.1.1 34354
-
 3 * 34354, calculation from sequence Pyrobaculum calidifontis
3.1.1.1 98000
-
 gel filtration Pyrobaculum calidifontis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 2-propanol 1 h, 80%, 6% loss of activity Pyrobaculum calidifontis
3.1.1.1 acetonitrile 1 h, 80%, no loss of activity Pyrobaculum calidifontis
3.1.1.1 dimethyl sulfoxide 1 h, 80%, 10% loss of activity Pyrobaculum calidifontis
3.1.1.1 dimethylformamide 1 h, 80%, 4% loss of activity Pyrobaculum calidifontis
3.1.1.1 Ethanol 1 h, 80%, no loss of activity Pyrobaculum calidifontis
3.1.1.1 Methanol 1 h, 80%,% loss of activity Pyrobaculum calidifontis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pyrobaculum calidifontis Q8NKS0
-
-
3.1.1.1 Pyrobaculum calidifontis VA1 Q8NKS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Pyrobaculum calidifontis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 160
-
 pH 7.1, 70°C, substrate: methyl acetate Pyrobaculum calidifontis
3.1.1.1 272
-
 pH 7.1, 70°C, substrate: tert-butyl acetate Pyrobaculum calidifontis
3.1.1.1 313
-
 pH 7.1, 70°C, substrate: ethyl acetate Pyrobaculum calidifontis
3.1.1.1 390
-
 pH 7.1, 70°C, substrate: isopropyl acetate Pyrobaculum calidifontis
3.1.1.1 559
-
 pH 7.1, 70°C, substrate: octyl acetate Pyrobaculum calidifontis
3.1.1.1 747
-
 pH 7.1, 70°C, substrate: hexyl acetate Pyrobaculum calidifontis
3.1.1.1 805
-
 pH 7.1, 70°C, substrate: propyl acetate Pyrobaculum calidifontis
3.1.1.1 876
-
 pH 7.1, 70°C, substrate: sec-butyl acetate Pyrobaculum calidifontis
3.1.1.1 1160
-
 pH 7.1, 70°C, substrate: butyl acetate Pyrobaculum calidifontis
3.1.1.1 1510
-
 pH 7.1, 70°C, substrate: isobutyl acetate Pyrobaculum calidifontis
3.1.1.1 4050
-
 pH 7.1, 70°C, substrate: 4-nitrophenyl hexanoate Pyrobaculum calidifontis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Pyrobaculum calidifontis 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Pyrobaculum calidifontis VA1 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Pyrobaculum calidifontis 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Pyrobaculum calidifontis 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Pyrobaculum calidifontis VA1 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Pyrobaculum calidifontis 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Pyrobaculum calidifontis 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Pyrobaculum calidifontis VA1 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl propanoate + H2O
-
 Pyrobaculum calidifontis 4-nitrophenol + propanoate
-
 ?
3.1.1.1 butyl acetate + H2O
-
 Pyrobaculum calidifontis butanol + acetate
-
 ?
3.1.1.1 ethyl acetate + H2O
-
 Pyrobaculum calidifontis ethanol + acetate
-
 ?
3.1.1.1 ethyl acetate + H2O
-
 Pyrobaculum calidifontis VA1 ethanol + acetate
-
 ?
3.1.1.1 isobutyl acetate + H2O
-
 Pyrobaculum calidifontis isobutanol + acetate
-
 ?
3.1.1.1 isopropyl acetate + H2O
-
 Pyrobaculum calidifontis isopropanol + acetate
-
 ?
3.1.1.1 methyl acetate + H2O
-
 Pyrobaculum calidifontis methanol + acetate
-
 ?
3.1.1.1 methyl acetate + H2O
-
 Pyrobaculum calidifontis VA1 methanol + acetate
-
 ?
3.1.1.1 octyl acetate + H2O
-
 Pyrobaculum calidifontis octanol + acetate
-
 ?
3.1.1.1 propyl acetate + H2O
-
 Pyrobaculum calidifontis propanol + acetate
-
 ?
3.1.1.1 sec-butyl acetate + H2O
-
 Pyrobaculum calidifontis sec-butanol + acetate
-
 ?
3.1.1.1 tert-butyl acetate + H2O
-
 Pyrobaculum calidifontis tert-butanol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 trimer 3 * 34000, SDS-PAGE Pyrobaculum calidifontis
3.1.1.1 trimer 3 * 34354, calculation from sequence Pyrobaculum calidifontis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 70
-
 assay at Pyrobaculum calidifontis
3.1.1.1 90
-
-
 Pyrobaculum calidifontis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 30 90 activity at 30°C is 16% of the maximum activity (at 90°C) Pyrobaculum calidifontis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 100
-
 2 h, stable Pyrobaculum calidifontis
3.1.1.1 110
-
 half-life: 56 min Pyrobaculum calidifontis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 1480
-
 4-nitrophenyl propanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 1600
-
 4-nitrophenyl octanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 1690
-
 4-nitrophenyl butanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 2070
-
 4-nitrophenyl pentanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 2620
-
 4-nitrophenyl hexanoate pH 7.1, 70°C Pyrobaculum calidifontis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.1
-
 assay at Pyrobaculum calidifontis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 4300
-
 4-nitrophenyl propanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 10000
-
 4-nitrophenyl butanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 29000
-
 4-nitrophenyl pentanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 31000
-
 4-nitrophenyl octanoate pH 7.1, 70°C Pyrobaculum calidifontis
3.1.1.1 59000
-
 4-nitrophenyl hexanoate pH 7.1, 70°C Pyrobaculum calidifontis