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Literature summary extracted from

  • Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Gumerov, V.M.; Artemova, N.; Kleymenov, S.Y.; Popov, V.O.
    Sodium chloride-induced modulation of the activity and thermal stability of short-chain oxidoreductase from the archaeon Thermococcus sibiricus (2013), Appl. Biochem. Biotechnol., 171, 1877-1889.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.71 expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal Thermococcus sibiricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.71 0.02
-
 NADP+ 60°C, pH 10.5, cosubstrate: 2,5-hexandiol Thermococcus sibiricus
1.1.1.71 0.02
-
 NADPH 60°C, pH 7.5, cosubstrate: pyruvaldehyde Thermococcus sibiricus
1.1.1.71 1.5
-
 2,3-Pentanedione 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 13.1
-
 (2S,5S)-2,5-hexanediol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 17.75
-
 Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 168
-
 2-propanol 60°C, pH 10.5 Thermococcus sibiricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.71 NaCl activity is increased in the presence of NaCl and remains at the elevated level up to 4 M of NaCl. The rates of 2-propanol and 2,5-hexanediol oxidation are increased by more than 2fold after the addition of NaCl up to 1 M to the assay mixture and is retained at the increased level up to 4 M of NaCl Thermococcus sibiricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Thermococcus sibiricus C6A190
-
-
1.1.1.71 Thermococcus sibiricus DSM 12597 C6A190
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.71 (2S,5S)-2,5-hexanediol + NADP+
-
 Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 (2S,5S)-2,5-hexanediol + NADP+
-
 Thermococcus sibiricus DSM 12597 ? + NADPH + H+
-
 ?
1.1.1.71 2,3-pentanedione + NADPH + H+
-
 Thermococcus sibiricus ? + NADP+
-
 ?
1.1.1.71 2,3-pentanedione + NADPH + H+
-
 Thermococcus sibiricus DSM 12597 ? + NADP+
-
 ?
1.1.1.71 2-propanol + NADP+
-
 Thermococcus sibiricus acetone + NADPH + H+
-
 ?
1.1.1.71 2-propanol + NADP+
-
 Thermococcus sibiricus DSM 12597 acetone + NADPH + H+
-
 ?
1.1.1.71 pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus lactaldehyde + NADP+
-
 ?
1.1.1.71 pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus DSM 12597 lactaldehyde + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.71 tetramer
-
 Thermococcus sibiricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.71 TsAdh319
-
 Thermococcus sibiricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.71 60
-
 assay at Thermococcus sibiricus
1.1.1.71 70
-
 oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl Thermococcus sibiricus
1.1.1.71 83
-
 oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl Thermococcus sibiricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.71 50 75 50°C: about 50% of maximal activity, 75°C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl Thermococcus sibiricus
1.1.1.71 65 86 65°C: about 50% of maximal activity, 86°C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl Thermococcus sibiricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.71 additional information
-
 thermal denaturation is fully irreversible and follows first-order kinetics Thermococcus sibiricus
1.1.1.71 80
-
 activity begins to decrease after 2 h preincubation. The rate of 2-propanol oxidation is decreased 34% and 58% over 24 h incubation at 80°C in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 37 vs. 20 h at 80°C, respectively Thermococcus sibiricus
1.1.1.71 90
-
 activity begins to decrease after 30 min preincubation. The incubation at 90°C provokes 31% and 40% reduction of 2-propanol oxidation rate in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 8 vs. 6 h at 90 °C, respectively Thermococcus sibiricus
1.1.1.71 102
-
 Tm-value in presence of 1 M NaCl Thermococcus sibiricus
1.1.1.71 104
-
 Tm-value in absence of NaCl Thermococcus sibiricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.71 0.53
-
 2-propanol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 1.4
-
 2,3-Pentanedione 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 1.51
-
 NADPH 60°C, pH 7.5, cosubstrate: pyruvaldehyde Thermococcus sibiricus
1.1.1.71 2.02
-
 NADP+ 60°C, pH 10.5, cosubstrate: 2,5-hexandiol Thermococcus sibiricus
1.1.1.71 2.04
-
 Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 2.2
-
 (2S,5S)-2,5-hexanediol 60°C, pH 10.5 Thermococcus sibiricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.71 7.5
-
 assay at Thermococcus sibiricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.71 0.0032
-
 2-propanol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.11
-
 Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 0.17
-
 (2S,5S)-2,5-hexanediol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 1.33
-
 2,3-Pentanedione 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 76
-
 NADPH 60°C, pH 7.5, cosubstrate: pyruvaldehyde Thermococcus sibiricus
1.1.1.71 101
-
 NADP+ 60°C, pH 10.5, cosubstrate: 2,5-hexandiol Thermococcus sibiricus