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Literature summary extracted from

  • Dalrymple, S.A.; Sheoran, I.; Kaminskyj, S.G.; Sanders, D.A.
    Preliminary X-ray crystallographic studies of UDP-glucose-4-epimerase from Aspergillus nidulans (2011), Acta Crystallogr. Sect. F, 67, 885-887.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) Aspergillus nidulans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.2 purified recombinant enzyme in complex with UDP-galactose using the microbatch method, 15 mg/ml protein in 25 mM Tris, pH 8, is used with crystallization solution containing 20% w/v PEG 3350, 0.1 M Bis-Tris propane, pH 7.5, and 0.2 M sodium fluoride, 2 weeks, X-ray diffraction structure determination and analysis at 2.4 A resolution Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.2 UDP-alpha-D-glucose Aspergillus nidulans the NAD+-dependent enzyme is responsible for reversibly inverting the 4-hydroxyl configuration of UDP-alpha-D-galactose to form UDP-alpha-D-glucose UDP-alpha-D-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Aspergillus nidulans C8VAU8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-alpha-D-glucose
-
 Aspergillus nidulans UDP-alpha-D-galactose
-
 r
5.1.3.2 UDP-alpha-D-glucose the NAD+-dependent enzyme is responsible for reversibly inverting the 4-hydroxyl configuration of UDP-alpha-D-galactose to form UDP-alpha-D-glucose Aspergillus nidulans UDP-alpha-D-galactose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 GalE
-
 Aspergillus nidulans
5.1.3.2 UDP-galactose-4-epimerase
-
 Aspergillus nidulans
5.1.3.2 UDP-glucose-4-epimerase
-
 Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.2 NAD+ dependent on Aspergillus nidulans