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Literature summary extracted from

  • Lee, E.H.; Lee, K.; Hwang, K.Y.
    Structural characterization and comparison of the large subunits of IPM isomerase and homoaconitase from Methanococcus jannaschii (2014), Acta Crystallogr. Sect. D, 70, 922-931.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.33 expression in Escherichia coli BL21(DE3) Star cells Methanocaldococcus jannaschii
4.2.1.36 expressed in Escherichia coli BL21(DE3) Star cells Methanocaldococcus jannaschii
4.2.1.114 expression in Escherichia coli BL21(DE3) Star cells Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.33 sitting-drop vapour-diffusion methodand hanging-drop vapour diffusion at 20°C, structures of oxidized and reduced forms of the large subunit of isopropylmalate isomerase (ox-MJ0499 and red-MJ0499, respectively) are reported at 1.8 and 2.7 A resolution, respectively. Significant large conformational changes are observed in the active site of red-MJ0499 when compared with ox-MJ0499 Methanocaldococcus jannaschii
4.2.1.36 sitting drop vapor diffusion method, small subunit, using 60% (w/v) 2-methyl-2,4-pentanediol and 0.1 M bicine pH 9.7 Methanocaldococcus jannaschii
4.2.1.114 sitting-drop vapour-diffusion method and hanging-drop vapour diffusion at 20°C, structures of the large subunit of homoaconise is reported at 2.5 A resolution Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.33 Iron-sulfur cluster the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site Methanocaldococcus jannaschii
4.2.1.114 Iron-sulfur cluster the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.33 Methanocaldococcus jannaschii P81291 large subunit of 3-isopropylmalate dehydratase
-
4.2.1.33 Methanocaldococcus jannaschii DSM 2661 P81291 large subunit of 3-isopropylmalate dehydratase
-
4.2.1.36 Methanocaldococcus jannaschii P81291
-
-
4.2.1.114 Methanocaldococcus jannaschii Q58409 large subunit of homoaconitase
-
4.2.1.114 Methanocaldococcus jannaschii DSM 2661 Q58409 large subunit of homoaconitase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.33
-
Methanocaldococcus jannaschii
4.2.1.36 HisTrap HP column chromatography and Superdex 200 gel filtration Methanocaldococcus jannaschii
4.2.1.114
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.36 cis-homoaconitate
-
Methanocaldococcus jannaschii homoisocitrate + H2O
-
?
4.2.1.36 cis-homoaconitate
-
Methanocaldococcus jannaschii DSM 2661 homoisocitrate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.36 dimer x-ray crystallography Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.2.1.33 isopropylmalate isomerase
-
Methanocaldococcus jannaschii
4.2.1.33 leuC
-
Methanocaldococcus jannaschii
4.2.1.33 MJ0499 gene name, large subunit of 3-isopropylmalate dehydratase Methanocaldococcus jannaschii
4.2.1.36 Homoaconitase
-
Methanocaldococcus jannaschii
4.2.1.36 leuC large subunit Methanocaldococcus jannaschii
4.2.1.36 MJ0499
-
Methanocaldococcus jannaschii
4.2.1.114 HacA
-
Methanocaldococcus jannaschii
4.2.1.114 Homoaconitase
-
Methanocaldococcus jannaschii
4.2.1.114 MJ1003 gene name, large subunit of homoaconitase Methanocaldococcus jannaschii