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Literature summary extracted from

  • Larson, E.T.; Kim, J.E.; Napuli, A.J.; Verlinde, C.L.; Fan, E.; Zucker, F.H.; Van Voorhis, W.C.; Buckner, F.S.; Hol, W.G.; Merritt, E.A.
    Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia (2012), Acta Crystallogr. Sect. D, 68, 1194-1200.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expressed in Escherichia coli Giardia intestinalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.15 sitting drop vapor diffusion method, using 28% (w/v) PEG 3350, 0.2 M magnesium chloride, 0.1 M Tris-HCl pH 7.8, at 4°C Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Giardia intestinalis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Giardia intestinalis WB / CC 30957
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Giardia intestinalis A8BR89 synonym Giardia lamblia
-
6.1.1.15 Giardia intestinalis WB / CC 30957 A8BR89 synonym Giardia lamblia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 Ni-NTA column chromatography and Superdex 75 gel filtration Giardia intestinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Giardia intestinalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Giardia intestinalis WB / CC 30957 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
 Giardia intestinalis
6.1.1.15 ProRS
-
 Giardia intestinalis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Giardia intestinalis