EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.18 | gene purE, expression of His-tagged enzyme in Escherichia coli strain BL21 Star (DE3) | Staphylococcus aureus |
6.3.4.18 | expressed in Escherichia coli BL21 Star (DE3) cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.4.99.18 | purified recombinant detagged enzyme, crystallization at room temperature, 10 mg/ml protein is mixed with a reservoir solution consisting of 0.1 M sodium acetate trihydrate, pH 4.5, and 2 M ammonium sulfate, 24 h, X-ray diffraction structure determination and analysis at 1.45 Aresolution, molecular replacement using the structure of Bacillus anthracis PurE, PDB ID 1xmp, as template | Staphylococcus aureus |
6.3.4.18 | hanging drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 2000 | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.18 | H38F | site-directed mutagenesis of the catalytic residue renders the enzyme inactive | Staphylococcus aureus |
5.4.99.18 | H38N | site-directed mutagenesis of the catalytic residue renders the enzyme inactive | Staphylococcus aureus |
5.4.99.18 | H38W | site-directed mutagenesis of the catalytic residue renders the enzyme inactive | Staphylococcus aureus |
6.3.4.18 | F78I | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
6.3.4.18 | F78W | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.18 | 0.0139 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 0.0432 | - |
ATP | wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 0.0513 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | mutant enzyme F78I, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 0.103 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | mutant enzyme F78W, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 18.8 | - |
HCO3- | wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.18 | Mg2+ | required | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.18 | 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | Staphylococcus aureus | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate | - |
? | |
6.3.4.18 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+ | Staphylococcus aureus | - |
ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
? | |
6.3.4.18 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+ | Staphylococcus aureus Newman | - |
ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.18 | Staphylococcus aureus | A0A0H3K7Y1 | gene purE | - |
6.3.4.18 | Staphylococcus aureus | A0A0H3KFM7 | - |
- |
6.3.4.18 | Staphylococcus aureus Newman | A0A0H3KFM7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.18 | recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration | Staphylococcus aureus |
6.3.4.18 | Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.18 | 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
Staphylococcus aureus | 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate | - |
? | |
6.3.4.18 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+ | - |
Staphylococcus aureus | ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
? | |
6.3.4.18 | ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- + H+ | - |
Staphylococcus aureus Newman | ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.18 | homodimer | x-ray crystallography | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.18 | N5-carboxyaminoimidazole ribonucleotide synthetase | - |
Staphylococcus aureus |
5.4.99.18 | PurE | - |
Staphylococcus aureus |
6.3.4.18 | N5-CAIR synthetase | - |
Staphylococcus aureus |
6.3.4.18 | N5-carboxyaminoimidazole ribonucleotide synthetase | - |
Staphylococcus aureus |
6.3.4.18 | PurK | - |
Staphylococcus aureus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.18 | 30 | - |
assay at | Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.18 | 15.83 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | mutant enzyme F78I, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 18 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | mutant enzyme F78W, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 24.83 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.18 | 7.8 | - |
assay at | Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.18 | ATP | - |
Staphylococcus aureus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.18 | additional information | His38 is essential for function, role of His38 as a general acid/base catalyst, structure analysis, comparison to Homo sapiens class II PurE enzyme, active site structure, overview | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.18 | 175 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | mutant enzyme F78W, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 308 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | mutant enzyme F78I, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus | |
6.3.4.18 | 1783 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole | wild type enzyme, in 50 mM HEPES, 80 mM KCl, 20 mM MgCl2, at 22°C, pH not specified in the publication | Staphylococcus aureus |