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Literature summary extracted from
- Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli (2012), Acta Biochim. Pol., 59, 425-431.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | locus DR-2036, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of C-terminally His6-tagged enzyme in Escherichia coli strain (DE3) Rosetta pLysS from pET30Ek/LIC vector | Deinococcus radiodurans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.16 | Al3+ | 98.5% inhibition at 1 mM | Deinococcus radiodurans |  |
| 5.4.99.16 | Ca2+ | 13.4% inhibition at 10 mM, 9.5% at 1 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Co2+ | 17.2% inhibition at 1 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Cu2+ | complete inhibition at 10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | dithiothreitol | 9% inhibition at 5 mM, 12% at 10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | EDTA | 15% inhibition at 1 mM, 35% at 10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Hg2+ | complete inhibition at 1 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Mg2+ | 8.5% inhibition at 1-10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Mn2+ | 20% inhibition at 1 mM, 30% at 10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Ni2+ | 28.5% inhibition at 1mM, 45% at 10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | SDS | 95.1% inhibition at 5 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Tris | 58% inhibition at 5 mM, 78% at 10 mM | Deinococcus radiodurans | |
| 5.4.99.16 | Zn2+ | complete inhibition at 5 mM | Deinococcus radiodurans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 290.7 | - |
maltose | pH 7.6, 30°C, recombinant His6-tagged enzyme | Deinococcus radiodurans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 62710 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
| 5.4.99.16 | 64000 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
| 5.4.99.16 | 64690 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
| 5.4.99.16 | 126900 | - |
recombinant His6-tagged enzyme, gel filtration | Deinococcus radiodurans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | Deinococcus radiodurans | - |
alpha,alpha-trehalose | - |
? | |
| 5.4.99.16 | maltose | Deinococcus radiodurans DSM Z 20539 | - |
alpha,alpha-trehalose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.16 | Deinococcus radiodurans | Q9RST7 | locus DR-2036 | - |
| 5.4.99.16 | Deinococcus radiodurans DSM Z 20539 | Q9RST7 | locus DR-2036 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain (DE3) Rosetta pLysS by cobalt affinity chromatography and ultrafiltration | Deinococcus radiodurans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | - |
Deinococcus radiodurans | alpha,alpha-trehalose | - |
? | |
| 5.4.99.16 | maltose | - |
Deinococcus radiodurans DSM Z 20539 | alpha,alpha-trehalose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | homodimer | 2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | Trehalose synthase | - |
Deinococcus radiodurans |
| 5.4.99.16 | TreS | - |
Deinococcus radiodurans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 30 | - |
- |
Deinococcus radiodurans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 5 | 50 | activity range, profile, overview | Deinococcus radiodurans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 45 | - |
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable up to | Deinococcus radiodurans |
| 5.4.99.16 | 50 | - |
purified recombinant His6-tagged enzyme, 2 h, pH 7.6, inactivation | Deinococcus radiodurans |
| 5.4.99.16 | 50 | - |
purified recombinant His6-tagged enzyme, 8 h, pH 7.6, 56% activity remaining | Deinococcus radiodurans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 7.6 | - |
- |
Deinococcus radiodurans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 4.5 | 9.5 | activity range, profile, overview | Deinococcus radiodurans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 4 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation | Deinococcus radiodurans |
| 5.4.99.16 | 5.5 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining | Deinococcus radiodurans |
| 5.4.99.16 | 7.6 | - |
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable | Deinococcus radiodurans |
| 5.4.99.16 | 9.5 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining | Deinococcus radiodurans |
| 5.4.99.16 | 10 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation | Deinococcus radiodurans |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.4.99.16 | Deinococcus radiodurans | sequence calculation | - |
4.94 |
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