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Literature summary extracted from

  • Iqbal, N.; Rudroff, F.; Brige, A.; Van Beeumen, J.; Mihovilovic, M.D.
    Asymmetric bioreduction of activated carbon-carbon double bonds using Shewanella yellow enzyme (SYE-4) as novel enoate reductase (2012), Tetrahedron, 68, 7619-7623.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.31 expressed in Escherichia coli BL21 Shewanella sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Shewanella sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 (+)-carvone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 97% Shewanella sp. ?
-
?
1.3.1.31 (-)-carvone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 95% Shewanella sp. ?
-
?
1.3.1.31 (2E)-2-benzylidenecyclohexanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 51% Shewanella sp. ?
-
?
1.3.1.31 (2E)-2-benzylidenecyclopentanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 4%, ee: racemate Shewanella sp. ?
-
?
1.3.1.31 1,3-dimethyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1.3.1.31 1-(cyclohex-1-en-1-yl)ethanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 71%, ee: not applicable Shewanella sp. ?
-
?
1.3.1.31 1-(cyclopent-1-en-1-yl)ethanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 30%, ee: not applicable Shewanella sp. ?
-
?
1.3.1.31 1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: not applicable Shewanella sp. ?
-
?
1.3.1.31 1-butyl-3-methyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1.3.1.31 1-ethyl-3-methyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1.3.1.31 2-methylcyclopent-2-enone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 52% Shewanella sp. ?
-
?
1.3.1.31 3-methyl-1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1.3.1.31 3-methyl-1-propyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1.3.1.31 diethyl (2Z)-2-methylbut-2-enedioate + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1.3.1.31 dimethyl (2Z)-2-methylbut-2-enedioate + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 98% Shewanella sp. ?
-
?
1.3.1.31 dimethyl 2-methylidenebutanedioate + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 19%, ee: 90% Shewanella sp. ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.31 enoate reductase
-
Shewanella sp.
1.3.1.31 SYE-4
-
Shewanella sp.
1.3.1.31 yellow enzyme
-
Shewanella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.31 28
-
assay at Shewanella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.31 8
-
assay at Shewanella sp.