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New insights into the half-of-the-sites reactivity of human aldehyde dehydrogenase 1A1

Yoval-Sanchez, B.; Pardo, J.P.; Rodriguez-Zavala, J.S.; Proteins 81, 1330-1339 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.3
the GFP-tagged enzyme is expressed in Escherichia coli BL21(DE3) pLysS cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.3
D80G/S82A
the unstable mutant starts to denature at urea concentrations below 0.5 M, reaching half of the denaturation at 1.2 M urea
Homo sapiens
General Stability
EC Number
General Stability
Organism
1.2.1.3
urea concentrations higher than 4.5 M almost completely denature wild type isoform ALDH1A1
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.3
acrolein
-
Homo sapiens
1.2.1.3
arsenite
47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4
Homo sapiens
1.2.1.3
Chloroacetaldehyde
86% residual activity at 1 mM chloroacetaldehyde at pH 9.5
Homo sapiens
1.2.1.3
Mg2+
wild type enzyme activity is inhibited (64%) by Mg2+ at pH 7.4, whereas the GFP-tagged enzyme activity is not affected by Mg2+
Homo sapiens
1.2.1.3
additional information
not influenced by acetaldehyde
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.3
0.0043
-
malondialdehyde
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.009
-
acrolein
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.011
-
NAD+
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.012
-
propionaldehyde
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.136
-
NAD+
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.189
-
propionaldehyde
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.595
-
glyceraldehyde
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.3
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.3
50000
-
4 * 50000, SDS-PAGE
Homo sapiens
1.2.1.3
85000
-
2 * 85000, GFP-tagged enzyme, SDS-PAGE
Homo sapiens
1.2.1.3
170000
-
GFP-tagged enzyme, gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.3
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.3
nickel chelating Sepharose column chromatography
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.3
acrolein + NAD+ + H2O
-
726464
Homo sapiens
? + NADH + H+
-
-
-
?
1.2.1.3
glyceraldehyde + NAD+ + H2O
-
726464
Homo sapiens
glycerate + NADH + H+
-
-
-
?
1.2.1.3
malondialdehyde + NAD+ + H2O
-
726464
Homo sapiens
? + NADH + H+
-
-
-
?
1.2.1.3
additional information
no activity with aromatic aldehydes such as benzaldehyde, nitrobenzaldehyde, methoxybenzaldehyde, or phenylacetaldehyde
726464
Homo sapiens
?
-
-
-
-
1.2.1.3
propionaldehyde + NAD+ + H2O
-
726464
Homo sapiens
propionate + NADH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.3
homodimer
2 * 85000, GFP-tagged enzyme, SDS-PAGE
Homo sapiens
1.2.1.3
homotetramer
4 * 50000, SDS-PAGE
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.3
NAD+
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.3
the GFP-tagged enzyme is expressed in Escherichia coli BL21(DE3) pLysS cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.3
NAD+
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.3
D80G/S82A
the unstable mutant starts to denature at urea concentrations below 0.5 M, reaching half of the denaturation at 1.2 M urea
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.3
urea concentrations higher than 4.5 M almost completely denature wild type isoform ALDH1A1
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.3
acrolein
-
Homo sapiens
1.2.1.3
arsenite
47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4
Homo sapiens
1.2.1.3
Chloroacetaldehyde
86% residual activity at 1 mM chloroacetaldehyde at pH 9.5
Homo sapiens
1.2.1.3
Mg2+
wild type enzyme activity is inhibited (64%) by Mg2+ at pH 7.4, whereas the GFP-tagged enzyme activity is not affected by Mg2+
Homo sapiens
1.2.1.3
additional information
not influenced by acetaldehyde
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.3
0.0043
-
malondialdehyde
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.009
-
acrolein
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.011
-
NAD+
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.012
-
propionaldehyde
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.136
-
NAD+
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.189
-
propionaldehyde
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
1.2.1.3
0.595
-
glyceraldehyde
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.3
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.3
50000
-
4 * 50000, SDS-PAGE
Homo sapiens
1.2.1.3
85000
-
2 * 85000, GFP-tagged enzyme, SDS-PAGE
Homo sapiens
1.2.1.3
170000
-
GFP-tagged enzyme, gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.3
nickel chelating Sepharose column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.3
acrolein + NAD+ + H2O
-
726464
Homo sapiens
? + NADH + H+
-
-
-
?
1.2.1.3
glyceraldehyde + NAD+ + H2O
-
726464
Homo sapiens
glycerate + NADH + H+
-
-
-
?
1.2.1.3
malondialdehyde + NAD+ + H2O
-
726464
Homo sapiens
? + NADH + H+
-
-
-
?
1.2.1.3
additional information
no activity with aromatic aldehydes such as benzaldehyde, nitrobenzaldehyde, methoxybenzaldehyde, or phenylacetaldehyde
726464
Homo sapiens
?
-
-
-
-
1.2.1.3
propionaldehyde + NAD+ + H2O
-
726464
Homo sapiens
propionate + NADH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.3
homodimer
2 * 85000, GFP-tagged enzyme, SDS-PAGE
Homo sapiens
1.2.1.3
homotetramer
4 * 50000, SDS-PAGE
Homo sapiens