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Literature summary extracted from

  • Aachmann, F.L.; Kwak, G.H.; Del Conte, R.; Kim, H.Y.; Gladyshev, V.N.; Dikiy, A.
    Structural and biochemical analysis of mammalian methionine sulfoxide reductase B2 (2011), Proteins, 79, 3123-3131.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.12 C98S the mutant show increased Km values with dithiothreitol (1.7fold) and thioredoxin (6fold) compared to the wild type enzyme Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.12 0.03
-
 dithiothreitol wild type enzyme, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus
1.8.4.12 0.05
-
 dithiothreitol mutant enzyme C98S, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus
1.8.4.12 0.66
-
 thioredoxin wild type enzyme, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus
1.8.4.12 7
-
 thioredoxin mutant enzyme C98S, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.12 mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.12 Zn2+ contains zinc Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Mus musculus Q78J03
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.12 0.023
-
 in 100 mM sodium phosphate, at 37°C and pH 5.7 Mus musculus
1.8.4.12 0.055
-
 in 100 mM sodium phosphate, at 37°C and pH 6.5 Mus musculus
1.8.4.12 0.074
-
 in 50 mM carbonate-bicarbonate, at 37°C and pH 10.0 Mus musculus
1.8.4.12 0.11
-
 in 100 mM sodium phosphate, at 37°C and pH 7.0 Mus musculus
1.8.4.12 0.119
-
 in 50 mM Tris-HCl, at 37°C and pH 7.6 Mus musculus
1.8.4.12 0.228
-
 in 100 mM sodium phosphate, at 37°C and pH 7.5 Mus musculus
1.8.4.12 0.267
-
 in 50 mM Tris-HCl, at 37°C and pH 8.0 Mus musculus
1.8.4.12 0.272
-
 in 50 mM carbonate-bicarbonate, at 37°C and pH 9.6 Mus musculus
1.8.4.12 0.446
-
 in 50 mM Tris-HCl, at 37°C and pH 8.6 Mus musculus
1.8.4.12 0.462
-
 in 50 mM carbonate-bicarbonate, at 37°C and pH 9.2 Mus musculus
1.8.4.12 0.484
-
 in 50 mM Tris-HCl, at 37°C and pH 9.0 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Mus musculus dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + thioredoxin
-
 Mus musculus dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 methionine sulfoxide reductase B2
-
 Mus musculus
1.8.4.12 MsrB2
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.12 9
-
-
 Mus musculus