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Literature summary extracted from

  • Liu, J.; Gerken, H.; Huang, J.; Chen, F.
    Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids (2013), Process Biochem., 48, 788-795.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.3.99.29 analysis the engineered endogenous CrPDS L505F mutant is a dominant selectable marker for Chlamydomonas reinhardtii and possibly for other green algae Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.29 gene pds, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3), quantitative real time PCR expression assay Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.29 L505F site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon Chlamydomonas reinhardtii
1.3.99.29 additional information possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae, overview Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.29 4-chloro-5-(methylamino)-2-[3-(trifluoromethyl)phenyl]pyridazin-3(2H)-one
-
Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.29 15-cis-phytoene + acceptor Chlamydomonas reinhardtii
-
all-trans-phytofluene + reduced acceptor
-
?
1.3.99.29 15-cis-phytoene + acceptor Chlamydomonas reinhardtii CC-1618
-
all-trans-phytofluene + reduced acceptor
-
?
1.3.99.29 all-trans-phytofluene + acceptor Chlamydomonas reinhardtii
-
all-trans-zeta-carotene + reduced acceptor
-
?
1.3.99.29 all-trans-phytofluene + acceptor Chlamydomonas reinhardtii CC-1618
-
all-trans-zeta-carotene + reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.29 Chlamydomonas reinhardtii Q6J213
-
-
1.3.99.29 Chlamydomonas reinhardtii CC-1618 Q6J213
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.29 15-cis-phytoene + acceptor
-
Chlamydomonas reinhardtii all-trans-phytofluene + reduced acceptor
-
?
1.3.99.29 15-cis-phytoene + acceptor
-
Chlamydomonas reinhardtii CC-1618 all-trans-phytofluene + reduced acceptor
-
?
1.3.99.29 15-cis-phytoene + decyl plastoquinone
-
Chlamydomonas reinhardtii all-trans-phytofluene + decyl plastoquinol
-
?
1.3.99.29 15-cis-phytoene + decyl plastoquinone
-
Chlamydomonas reinhardtii CC-1618 all-trans-phytofluene + decyl plastoquinol
-
?
1.3.99.29 all-trans-phytofluene + acceptor
-
Chlamydomonas reinhardtii all-trans-zeta-carotene + reduced acceptor
-
?
1.3.99.29 all-trans-phytofluene + acceptor
-
Chlamydomonas reinhardtii CC-1618 all-trans-zeta-carotene + reduced acceptor
-
?
1.3.99.29 all-trans-phytofluene + decyl plastoquinone
-
Chlamydomonas reinhardtii all-trans-zeta-carotene + decyl plastoquinol
-
?
1.3.99.29 all-trans-phytofluene + decyl plastoquinone
-
Chlamydomonas reinhardtii CC-1618 all-trans-zeta-carotene + decyl plastoquinol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.29 PDS
-
Chlamydomonas reinhardtii
1.3.99.29 phytoene desaturase
-
Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.29 28
-
assay at Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.29 7.2
-
assay at Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.29 decyl-plastoquinone
-
Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
1.3.99.29 additional information expression of mutant CrPDS-L505F enhances the accumulation of carotenoids, overview Chlamydomonas reinhardtii