EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.99.29 | analysis | the engineered endogenous CrPDS L505F mutant is a dominant selectable marker for Chlamydomonas reinhardtii and possibly for other green algae | Chlamydomonas reinhardtii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.29 | gene pds, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3), quantitative real time PCR expression assay | Chlamydomonas reinhardtii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.99.29 | L505F | site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon | Chlamydomonas reinhardtii |
1.3.99.29 | additional information | possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae, overview | Chlamydomonas reinhardtii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.29 | 4-chloro-5-(methylamino)-2-[3-(trifluoromethyl)phenyl]pyridazin-3(2H)-one | - |
Chlamydomonas reinhardtii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.29 | 15-cis-phytoene + acceptor | Chlamydomonas reinhardtii | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.29 | 15-cis-phytoene + acceptor | Chlamydomonas reinhardtii CC-1618 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.29 | all-trans-phytofluene + acceptor | Chlamydomonas reinhardtii | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.29 | all-trans-phytofluene + acceptor | Chlamydomonas reinhardtii CC-1618 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.29 | Chlamydomonas reinhardtii | Q6J213 | - |
- |
1.3.99.29 | Chlamydomonas reinhardtii CC-1618 | Q6J213 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.29 | 15-cis-phytoene + acceptor | - |
Chlamydomonas reinhardtii | all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.29 | 15-cis-phytoene + acceptor | - |
Chlamydomonas reinhardtii CC-1618 | all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.29 | 15-cis-phytoene + decyl plastoquinone | - |
Chlamydomonas reinhardtii | all-trans-phytofluene + decyl plastoquinol | - |
? | |
1.3.99.29 | 15-cis-phytoene + decyl plastoquinone | - |
Chlamydomonas reinhardtii CC-1618 | all-trans-phytofluene + decyl plastoquinol | - |
? | |
1.3.99.29 | all-trans-phytofluene + acceptor | - |
Chlamydomonas reinhardtii | all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.29 | all-trans-phytofluene + acceptor | - |
Chlamydomonas reinhardtii CC-1618 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.29 | all-trans-phytofluene + decyl plastoquinone | - |
Chlamydomonas reinhardtii | all-trans-zeta-carotene + decyl plastoquinol | - |
? | |
1.3.99.29 | all-trans-phytofluene + decyl plastoquinone | - |
Chlamydomonas reinhardtii CC-1618 | all-trans-zeta-carotene + decyl plastoquinol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.99.29 | PDS | - |
Chlamydomonas reinhardtii |
1.3.99.29 | phytoene desaturase | - |
Chlamydomonas reinhardtii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.29 | 28 | - |
assay at | Chlamydomonas reinhardtii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.99.29 | 7.2 | - |
assay at | Chlamydomonas reinhardtii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.29 | decyl-plastoquinone | - |
Chlamydomonas reinhardtii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.99.29 | additional information | expression of mutant CrPDS-L505F enhances the accumulation of carotenoids, overview | Chlamydomonas reinhardtii |