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Literature summary extracted from

  • Yang, Y.; Ding, Y.; Liao, X.; Cai, Y.
    Purification and characterization of a new laccase from Shiraia sp.SUPER-H168 (2013), Process Biochem., 48, 351-357.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 acetone
-
 Shiraia sp. SUPER-H168
1.10.3.2 Ag+ 8.13% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 DTT 43% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 EDTA 16% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 EGTA 22% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 ethanol
-
 Shiraia sp. SUPER-H168
1.10.3.2 Fe2+ complete inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 Fe3+ 50% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 glycerol
-
 Shiraia sp. SUPER-H168
1.10.3.2 isoamyl alcohol
-
 Shiraia sp. SUPER-H168
1.10.3.2 Isopropyl alcohol
-
 Shiraia sp. SUPER-H168
1.10.3.2 L-cysteine 44% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 methanol
-
 Shiraia sp. SUPER-H168
1.10.3.2 n-heptane
-
 Shiraia sp. SUPER-H168
1.10.3.2 n-hexane
-
 Shiraia sp. SUPER-H168
1.10.3.2 NaF 47% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 NaN3 7.86% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 SDS 10.2% inhibition at 10 mM Shiraia sp. SUPER-H168
1.10.3.2 tertiary butanol
-
 Shiraia sp. SUPER-H168

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.04
-
 syringaldazine pH 6.0, 50°C Shiraia sp. SUPER-H168
1.10.3.2 0.19
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 50°C Shiraia sp. SUPER-H168
1.10.3.2 0.37
-
 2,6-dimethoxyphenol pH 4.0, 50°C Shiraia sp. SUPER-H168
1.10.3.2 0.38
-
 guaiacol pH 5.5, 50°C Shiraia sp. SUPER-H168

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 additional information no or poor effect on enzyme activity by K+, Ca2+, Cu2+, Mg2+, Mn2+, Zn2+, and Ba2+ at 10 mM Shiraia sp. SUPER-H168

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 69000
-
 x * 70780, mass spectrometry, x * 69000, SDS-PAGE Shiraia sp. SUPER-H168
1.10.3.2 70780
-
 x * 70780, mass spectrometry, x * 69000, SDS-PAGE Shiraia sp. SUPER-H168

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 Acetone slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 Ethanol slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 glycer0l slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 isoamyl alcohol slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 isopropyl alcohol slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 Methanol slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 n-heptane slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 n-hexane slight loss of activity at 10% v/v Shiraia sp. SUPER-H168
1.10.3.2 tert-Butanol slight loss of activity at 10% v/v Shiraia sp. SUPER-H168

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Shiraia sp. SUPER-H168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 2.14fold by cation exchange chromatography and gel filtration Shiraia sp. SUPER-H168

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 417.7
-
 purified enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 50°C Shiraia sp. SUPER-H168

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Shiraia sp. SUPER-H168 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Shiraia sp. SUPER-H168 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Shiraia sp. SUPER-H168 ?
-
 ?
1.10.3.2 additional information approximately 66% of the Acid Blue 129 at 100 mg/l is decolorized by 2.5 U of the purified laccase after a 120 min incubation at 50°C, Acid Red 1 at 20 mg/l and Reactive Black 5 at 50 mg/l are decolorized by the purified laccase after the addition of Acid Blue 129 at 100 mg/l. Shiraia sp. SUPER-H168 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Shiraia sp. SUPER-H168 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 70780, mass spectrometry, x * 69000, SDS-PAGE Shiraia sp. SUPER-H168

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
 with substrates 2,6-dimethoxyphenol, syringaldazine, and guaiacol Shiraia sp. SUPER-H168
1.10.3.2 60
-
 with substrate 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) Shiraia sp. SUPER-H168

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 30.44
-
 guaiacol pH 5.5, 50°C Shiraia sp. SUPER-H168
1.10.3.2 938.1
-
 syringaldazine pH 6.0, 50°C Shiraia sp. SUPER-H168
1.10.3.2 1476
-
 2,6-dimethoxyphenol pH 4.0, 50°C Shiraia sp. SUPER-H168
1.10.3.2 7109
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 50°C Shiraia sp. SUPER-H168

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 with substrate 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) Shiraia sp. SUPER-H168
1.10.3.2 4
-
 with substrate 2,6-dimethoxyphenol Shiraia sp. SUPER-H168
1.10.3.2 5.5
-
 with substrate guaiacol Shiraia sp. SUPER-H168
1.10.3.2 6
-
 with substrate syringaldazine Shiraia sp. SUPER-H168

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6 7 purified enzyme, the enzyme retains 80% of its initial activity after 96 h Shiraia sp. SUPER-H168