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Literature summary extracted from

  • Akhtar, M.K.; Turner, N.J.; Jones, P.R.
    Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities (2013), Proc. Natl. Acad. Sci. USA, 110, 87-92.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.30 synthesis by combining the carboxylic acid reductase-dependent pathway with an exogenous fatty acid-generating lipase, natural oils (coconut oil, palm oil, and algal oil bodies) can be enzymatically converted into fatty alcohols across a broad chain length range (C8-C18) Mycobacterium marinum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.30 expressed in Escherichia coli BL21(DE3) cells Mycobacterium marinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.30 0.048
-
 NADPH pH and temperature not specified in the publication Mycobacterium marinum
1.2.1.30 0.115
-
 ATP pH and temperature not specified in the publication Mycobacterium marinum
1.2.1.30 0.362
-
 benzoic acid pH and temperature not specified in the publication Mycobacterium marinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.30 fatty acid + ATP + NADPH + H+ Mycobacterium marinum
-
 fatty aldehyde + AMP + diphosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.30 Mycobacterium marinum B2HN69
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.30 nickel affinity column chromatography Mycobacterium marinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.30 benzoic acid + ATP + NADPH + H+ highest turnover number Mycobacterium marinum benzaldehyde + AMP + diphosphate + NADP+
-
 ?
1.2.1.30 butyric acid + ATP + NADPH + H+
-
 Mycobacterium marinum butyraldehyde + AMP + diphosphate + NADP+ highest Km value ?
1.2.1.30 capric acid + ATP + NADPH + H+
-
 Mycobacterium marinum capraldehyde + AMP + diphosphate + NADP+
-
 ?
1.2.1.30 caproic acid + ATP + NADPH + H+
-
 Mycobacterium marinum caproaldehyde + AMP + diphosphate + NADP+
-
 ?
1.2.1.30 caprylic acid + ATP + NADPH + H+
-
 Mycobacterium marinum caprylaldehyde + AMP + diphosphate + NADP+
-
 ?
1.2.1.30 fatty acid + ATP + NADPH + H+
-
 Mycobacterium marinum fatty aldehyde + AMP + diphosphate + NADP+
-
 ?
1.2.1.30 lauric acid + ATP + NADPH + H+ highest catalytic efficiency Mycobacterium marinum lauraldehyde + AMP + diphosphate + NADP+
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.30 26 37 in vitro half-lives of 73, 70, and 48 h at 26, 30, and 37°C Mycobacterium marinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.30 7.5
-
-
 Mycobacterium marinum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.30 NADPH the enzyme has a selective preference for NADPH over NADH Mycobacterium marinum