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Literature summary extracted from

  • Steffler, F.; Sieber, V.
    Refolding of a thermostable glyceraldehyde dehydrogenase for application in synthetic cascade biomanufacturing (2013), PLoS One, 8, e70592.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.89 synthesis the enzyme accept NAD+ as cofactor under technically relevant conditions, making it suitable for application in the artificial glycolysis by synthetic cascade biomanufacturing Thermoplasma acidophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.89 the gene of TaAlDH is synthesized and cloned into a T7 based vector system for recombinant expression in Escherichia coli. Using matrix-assisted refolding of inclusion bodies the yield of enzyme production is enhanced 43fold Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.89 0.022
-
 NADP+ pH 6.2, 50°C Thermoplasma acidophilum

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.2.1.89 guanidine-HCl the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C Thermoplasma acidophilum
1.2.1.89 isobutanol the stability of the enzyme is ca. 28 kJ/mol both at 25°C and 40°C Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.89 Thermoplasma acidophilum Q9HK01
-
-
1.2.1.89 Thermoplasma acidophilum JCM 9062 Q9HK01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.89
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.89 D-glyceraldehyde + NADP+ + H2O also accepts NAD+ under technically relevant conditions, cofactor above 1 mM Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O also accepts NAD+ under technically relevant conditions, cofactor above 1 mM Thermoplasma acidophilum JCM 9062 D-glycerate + NADPH + H+
-
 ?
1.2.1.89 additional information no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose Thermoplasma acidophilum ?
-
 ?
1.2.1.89 additional information no activity with acetaldehyde, propionaldehyde, n-butyraldehyde, pyruvaldehyde, isobutyraldehyde or D-glucose Thermoplasma acidophilum JCM 9062 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.89 Ta0809 locus name Thermoplasma acidophilum
1.2.1.89 TaAlDH
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.89 50
-
 assay at Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.89 6.2
-
 assay at Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.89 NADP+ also accepts NAD+ under technically relevant conditions, cofactor above 1 mM, Km for NADP+: 0.022 mM, Km for NAD+ is 22.13 mM, at H 6.2, 50°C Thermoplasma acidophilum