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Literature summary extracted from

  • Song, L.; Li, J.; Xie, S.; Qian, C.; Wang, J.; Zhang, W.; Yin, X.; Hua, Z.; Yu, C.
    Thioredoxin glutathione reductase as a novel drug target: evidence from Schistosoma japonicum (2012), PLoS ONE, 7, e31456.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.8.1.B1 medicine the enzyme is a potential target for development of novel agents against schistosomes Schistosoma japonicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.B1 expressed in Escherichia coli BL21 (DE3) cells Schistosoma japonicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.B1 auranofin auranofin at 0.005 mg/ml (0.0074 mM) reduces 57.1% of the thioredoxin reductase activity and 71% of the glutathione reductase activity in the Schistosoma japonicum adult worms Schistosoma japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.00324
-
 thioredoxin disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 0.01097
-
 NADPH with glutaredoxin as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 0.02142
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 0.04955
-
 GSSG at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 0.08324
-
 NADPH with GSSG as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 0.1451
-
 5,5'-dithiobis(2-nitrobenzoic acid) at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 1.698
-
 GSH at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 2.792
-
 2-hydroxyethyl disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.B1 additional information selenoprotein Schistosoma japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.B1 64900
-
 x * 64900, calculated from amino acid sequence Schistosoma japonicum
1.8.1.B1 65000
-
 x * 65000, SDS-PAGE Schistosoma japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.B1 2 GSH + NADP+ Schistosoma japonicum
-
 GSSG + NADPH + H+
-
 ?
1.8.1.B1 GSSG + NADPH + H+ Schistosoma japonicum
-
 GSH + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+ Schistosoma japonicum
-
 thioredoxin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Schistosoma japonicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.B1 ADP-agarose column chromatography Schistosoma japonicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.B1 2.19
-
 with GSSG as substrate, at pH 7.4 and 25°C Schistosoma japonicum
1.8.1.B1 3.33
-
 with thioredoxin disulfide as substrate, at pH 7.4 and 25°C Schistosoma japonicum
1.8.1.B1 8.11
-
 with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.4 and 25°C Schistosoma japonicum
1.8.1.B1 12.1
-
 with HED as substrate, at pH 7.4 and 25°C Schistosoma japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 2 GSH + NADP+
-
 Schistosoma japonicum GSSG + NADPH + H+
-
 ?
1.8.1.B1 2-hydroxyethyl disulfide + NADPH + H+
-
 Schistosoma japonicum ?
-
 ?
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Schistosoma japonicum 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.B1 glutaredoxin + NADPH + H+
-
 Schistosoma japonicum ?
-
 ?
1.8.1.B1 GSSG + NADPH + H+
-
 Schistosoma japonicum GSH + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+
-
 Schistosoma japonicum thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.B1 ? x * 65000, SDS-PAGE Schistosoma japonicum
1.8.1.B1 ? x * 64900, calculated from amino acid sequence Schistosoma japonicum

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 TGR
-
 Schistosoma japonicum
1.8.1.B1 thioredoxin-glutathione reductase
-
 Schistosoma japonicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.B1 4.78
-
 thioredoxin disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 4.87
-
 NADPH with GSSG as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 5.35
-
 GSSG at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 8.16
-
 5,5'-dithiobis(2-nitrobenzoic acid) at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 8.65
-
 2-hydroxyethyl disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 10.92
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 14.93
-
 NADPH with glutaredoxin as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 15.78
-
 GSH at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.B1 NADPH
-
 Schistosoma japonicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.B1 Schistosoma japonicum calculated from amino acid sequence
-
 6.4

General Information

EC Number General Information Comment Organism
1.8.1.B1 physiological function the enzyme is essential for maintaining the thiol-disulfide redox homeostasis of Schistosoma japonicum Schistosoma japonicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.B1 3.1
-
 2-hydroxyethyl disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 9.3
-
 GSH at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 58.5
-
 NADPH with GSSG as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 108
-
 GSSG at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 119
-
 5,5'-dithiobis(2-nitrobenzoic acid) at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 510
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 1360
-
 NADPH with glutaredoxin as cosubstrate, at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum
1.8.1.B1 1480
-
 thioredoxin disulfide at 25°C in 0.1 M potassium phosphate (pH 7.4) Schistosoma japonicum