Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mashalidis, E.H.; Mukherjee, T.; Sled?, P.; Matak-Vinkovi?, D.; Boshoff, H.; Abell, C.; Barry, C.E.
    Rv2607 from Mycobacterium tuberculosis is a pyridoxine 5-phosphate oxidase with unusual substrate specificity (2011), PLoS ONE, 6, e27643.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.5 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.36
-
 pyridoxine 5'-phosphate in 25 mM potassium phosphate, pH 7.8 and 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 27000
-
 2 * 27000, SDS-PAGE Mycobacterium tuberculosis
1.4.3.5 54000
-
 gel filtration Mycobacterium tuberculosis
1.4.3.5 55380
-
 nano-ESI mass spectroscopy Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.5 additional information Mycobacterium tuberculosis the enzyme does not recognize pyridoxamine 5'-phosphate as substrate ?
-
 ?
1.4.3.5 additional information Mycobacterium tuberculosis H37Rv the enzyme does not recognize pyridoxamine 5'-phosphate as substrate ?
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + H2O + O2 Mycobacterium tuberculosis
-
 pyridoxal 5'-phosphate + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + H2O + O2 Mycobacterium tuberculosis H37Rv
-
 pyridoxal 5'-phosphate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Mycobacterium tuberculosis
-
-
-
1.4.3.5 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 additional information the enzyme does not recognize pyridoxamine 5'-phosphate as substrate Mycobacterium tuberculosis ?
-
 ?
1.4.3.5 additional information the enzyme does not recognize pyridoxamine 5'-phosphate as substrate Mycobacterium tuberculosis H37Rv ?
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + H2O + O2
-
 Mycobacterium tuberculosis pyridoxal 5'-phosphate + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + H2O + O2
-
 Mycobacterium tuberculosis H37Rv pyridoxal 5'-phosphate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 homodimer 2 * 27000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 PNPOx
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.5 0.01
-
 pyridoxine 5'-phosphate in 25 mM potassium phosphate, pH 7.8 and 25°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN contains one FMN molecule per dimer Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.5 0.028
-
 pyridoxine 5'-phosphate in 25 mM potassium phosphate, pH 7.8 and 25°C Mycobacterium tuberculosis