EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.20 | expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.1.20 | hanging drop vapor diffusion method, using 0.1 M sodium acetate buffer (NaOAc, pH 4.3,4.5), 1 M lithium chloride, 10% (w/v) polyethylene glycol 6000, 10-20% (v/v) glycerol, and 2-5% (v/v) dioxane, at 20°C | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.20 | A222V | the mutant shows enhanced instability upon loss of FAD | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.20 | 0.18 | - |
(6R)-5,10-methylenetetrahydrofolate | at pH 7.0 and 50°C | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | (6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+ | Thermus thermophilus | - |
(6S)-5-methyltetrahydrofolate + NAD(P)+ | - |
r | |
1.5.1.20 | (6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+ | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
(6S)-5-methyltetrahydrofolate + NAD(P)+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Thermus thermophilus | Q5SLG6 | - |
- |
1.5.1.20 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SLG6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.20 | Toyopearl SuperQ-650M column chromatography and nickel affinity column chromatography | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | (6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+ | - |
Thermus thermophilus | (6S)-5-methyltetrahydrofolate + NAD(P)+ | - |
r | |
1.5.1.20 | (6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+ | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | (6S)-5-methyltetrahydrofolate + NAD(P)+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.20 | heterodimer | x-ray crystallography | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.20 | MTHFR | - |
Thermus thermophilus |
1.5.1.20 | NADH:CH2-H4folate oxidoreductase | - |
Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | FAD | the enzyme contains one FAD prosthetic group bound per dimer. Km for FAD is 0.005 mM. The enzyme activity of the FAD-replete enzyme is approximately 50% compared to the normal purified enzyme | Thermus thermophilus | |
1.5.1.20 | NADH | - |
Thermus thermophilus | |
1.5.1.20 | NADPH | - |
Thermus thermophilus |