EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.60 | expression in Escherichia coli | Ustilago maydis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.60 | EDTA | severe inhibition | Ustilago maydis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.60 | 0.00019 | - |
2-hydroxy-3-oxopropanoate | pH 8.5, 40°C | Ustilago maydis | |
1.1.1.60 | 0.0003 | - |
NADP+ | pH 8.5, 40°C | Ustilago maydis | |
1.1.1.60 | 0.0143 | - |
NADH | pH 8.5, 40°C | Ustilago maydis | |
1.1.1.60 | 0.0177 | - |
D-glycerate | pH 8.5, 40°C | Ustilago maydis | |
1.1.1.60 | 0.123 | - |
L-Glycerate | pH 8.5, 40°C | Ustilago maydis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.60 | 40000 | - |
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein | Ustilago maydis |
1.1.1.60 | 41000 | - |
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein | Ustilago maydis |
1.1.1.60 | 85000 | - |
gel filtration | Ustilago maydis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.60 | Ustilago maydis | A0A0D1DYW4 | putative | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.60 | 4°C, phosphate buffer, pH 7.0, improved solubility | Ustilago maydis |
1.1.1.60 | 4°C, Tris-HCl, pH 8.0, highly unstable, precipitates during storage | Ustilago maydis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.60 | 2-hydroxy-3-oxopropanoate + NADH + H+ | - |
Ustilago maydis | D-glycerate + NAD+ | - |
? | |
1.1.1.60 | D-glycerate + NADP+ | enzyme shows strong specificity and enantioselectivity for D-glycerate | Ustilago maydis | 2-hydroxy-3-oxopropanoate + NADPH + H+ | - |
? | |
1.1.1.60 | L-glycerate + NADP+ | enzyme shows strong specificity and enantioselectivity for D-glycerate | Ustilago maydis | 2-hydroxy-3-oxopropanoate + NADPH + H+ | - |
? | |
1.1.1.60 | additional information | poor activity towards 6-phosphogluconic acid, beta-hydroxybutyric acid and D-threonine | Ustilago maydis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.60 | dimer | 2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein | Ustilago maydis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.60 | TSR1 | - |
Ustilago maydis |
1.1.1.60 | UM02592 | - |
Ustilago maydis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.60 | 40 | - |
- |
Ustilago maydis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.60 | 8.5 | - |
- |
Ustilago maydis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.60 | 6.5 | - |
rapidly precipitates below | Ustilago maydis |
1.1.1.60 | 7 | - |
phosphate buffer, pH 7.0, improved solubility | Ustilago maydis |
1.1.1.60 | 8 | - |
Tris-HCl, highly unstable, precipitates during storage | Ustilago maydis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.60 | NAD+ | about 75% of the activity with NADP+ | Ustilago maydis | |
1.1.1.60 | NADP+ | preferred over NAD+ | Ustilago maydis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.60 | physiological function | over-expression of the gene results in improved glycerol assimilation. Glycolipid accumulation is reduced by 45.2% in the knockout mutant whereas over-expression of the gene increases it by 40.4% | Ustilago maydis |