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Literature summary extracted from

  • Liu, Y.; Koh, C.; Sun, L.; Ji, L.
    Tartronate semialdehyde reductase defines a novel rate-limiting step in assimilation and bioconversion of glycerol in Ustilago maydis (2011), PLoS ONE, 6, e16438.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.60 expression in Escherichia coli Ustilago maydis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.60 EDTA severe inhibition Ustilago maydis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.60 0.00019
-
2-hydroxy-3-oxopropanoate pH 8.5, 40°C Ustilago maydis
1.1.1.60 0.0003
-
NADP+ pH 8.5, 40°C Ustilago maydis
1.1.1.60 0.0143
-
NADH pH 8.5, 40°C Ustilago maydis
1.1.1.60 0.0177
-
D-glycerate pH 8.5, 40°C Ustilago maydis
1.1.1.60 0.123
-
L-Glycerate pH 8.5, 40°C Ustilago maydis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.60 40000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein Ustilago maydis
1.1.1.60 41000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein Ustilago maydis
1.1.1.60 85000
-
gel filtration Ustilago maydis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.60 Ustilago maydis A0A0D1DYW4 putative
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.60 4°C, phosphate buffer, pH 7.0, improved solubility Ustilago maydis
1.1.1.60 4°C, Tris-HCl, pH 8.0, highly unstable, precipitates during storage Ustilago maydis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.60 2-hydroxy-3-oxopropanoate + NADH + H+
-
Ustilago maydis D-glycerate + NAD+
-
?
1.1.1.60 D-glycerate + NADP+ enzyme shows strong specificity and enantioselectivity for D-glycerate Ustilago maydis 2-hydroxy-3-oxopropanoate + NADPH + H+
-
?
1.1.1.60 L-glycerate + NADP+ enzyme shows strong specificity and enantioselectivity for D-glycerate Ustilago maydis 2-hydroxy-3-oxopropanoate + NADPH + H+
-
?
1.1.1.60 additional information poor activity towards 6-phosphogluconic acid, beta-hydroxybutyric acid and D-threonine Ustilago maydis ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.60 dimer 2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein Ustilago maydis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.60 TSR1
-
Ustilago maydis
1.1.1.60 UM02592
-
Ustilago maydis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.60 40
-
-
Ustilago maydis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.60 8.5
-
-
Ustilago maydis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.60 6.5
-
rapidly precipitates below Ustilago maydis
1.1.1.60 7
-
phosphate buffer, pH 7.0, improved solubility Ustilago maydis
1.1.1.60 8
-
Tris-HCl, highly unstable, precipitates during storage Ustilago maydis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.60 NAD+ about 75% of the activity with NADP+ Ustilago maydis
1.1.1.60 NADP+ preferred over NAD+ Ustilago maydis

General Information

EC Number General Information Comment Organism
1.1.1.60 physiological function over-expression of the gene results in improved glycerol assimilation. Glycolipid accumulation is reduced by 45.2% in the knockout mutant whereas over-expression of the gene increases it by 40.4% Ustilago maydis