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Literature summary extracted from

  • Mitsuya, S.; Kuwahara, J.; Ozaki, K.; Saeki, E.; Fujiwara, T.; Takabe, T.
    Isolation and characterization of a novel peroxisomal choline monooxygenase in barley (2011), Planta, 234, 1215-1226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.7 gene HvCMO, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR expression analyis, recombinant expression in Saccharomyces cerevisiae strain BJ5458 Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.7 additional information no activity in mitochondria and the endoplasmic reticulum-containing microsome fraction, as well as chloroplasts Hordeum vulgare
-
-
1.14.15.7 peroxisome
-
 Hordeum vulgare 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.7 Fe2+ in ferredoxin cofactor and a Rieske-type [2Fe-2S] cluster Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.7 44800
-
 x * 44800, about, sequence calculation Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.7 choline + O2 + 2 reduced ferredoxin + 2 H+ Hordeum vulgare
-
 betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.7 Hordeum vulgare B5UAE1 cv. Haruna-nijyo
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.7 awn
-
 Hordeum vulgare
-
1.14.15.7 floret
-
 Hordeum vulgare
-
1.14.15.7 internode
-
 Hordeum vulgare
-
1.14.15.7 leaf high content of glycine betaine, more in the older leaf blades, but little in the florets and internodes Hordeum vulgare
-
1.14.15.7 seedling
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.7 choline + O2 + 2 reduced ferredoxin + 2 H+
-
 Hordeum vulgare betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.7 ? x * 44800, about, sequence calculation Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
1.14.15.7 CMO
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.7 25
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.7 8
-
 assay at Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.7 Fe-S center Rieske-type [2Fe-2S] cluster Hordeum vulgare
1.14.15.7 Ferredoxin dependent on Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.15.7 Hordeum vulgare sequence calculation
-
 6.2

Expression

EC Number Organism Comment Expression
1.14.15.7 Hordeum vulgare the expression of HvCMO protein is increased by the presence of high NaCl, accumulation of HvCMO mRNA is increased by high osmotic and low-temperature environments up

General Information

EC Number General Information Comment Organism
1.14.15.7 metabolism the enzyme catalyzes the first stepin biosynthesis of glycine betaine. The second step is catalyzed by betaine aldehyde dehydrogenase Hordeum vulgare