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Literature summary extracted from

  • Thill, J.; Miosic, S.; Gotame, T.P.; Mikulic-Petkovsek, M.; Gosch, C.; Veberic, R.; Preuss, A.; Schwab, W.; Stampar, F.; Stich, K.; Halbwirth, H.
    Differential expression of flavonoid 3-hydroxylase during fruit development establishes the different B-ring hydroxylation patterns of flavonoids in Fragaria x ananassa and Fragaria vesca (2013), Plant Physiol. Biochem., 72, 72-78.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.82 0.0001
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.001
-
 naringenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.004
-
 naringenin pH 7.0, 25°C Fragaria vesca
1.14.14.82 0.009
-
 naringenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.021
-
 naringenin pH 7.0, 25°C Fragaria vesca
1.14.14.82 0.022
-
 naringenin pH 7.0, 25°C Fragaria vesca
1.14.14.82 0.048
-
 apigenin pH 7.0, 25°C Fragaria vesca

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.82 Fragaria vesca T1NXM1
-
-
1.14.14.82 Fragaria x ananassa T1NXM4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria vesca luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria vesca dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria vesca quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria vesca eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.82 25
-
-
 Fragaria x ananassa
1.14.14.82 30
-
-
 Fragaria vesca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.82 7
-
-
 Fragaria x ananassa
1.14.14.82 7
-
-
 Fragaria vesca

Expression

EC Number Organism Comment Expression
1.14.14.82 Fragaria x ananassa drastic decline in expression during fruit ripening down
1.14.14.82 Fragaria vesca enzyme is highly expressed in all stages of fruit ripening additional information

General Information

EC Number General Information Comment Organism
1.14.14.82 metabolism the drastic decline of flavonoid 3'-hydroxylase expression occurring during fruit ripening in Fragaria ananassa is reflected by the anthocyanin composition, which shows a prevalence of pelargonidin in ripe fruits of Fragaria ananassa Fragaria x ananassa
1.14.14.82 metabolism the high expression of flavonoid 3'-hydroxylase during fruit ripening in Fragaria vesca is reflected by the anthocyanin composition, which shows a high content of cyanidin Fragaria vesca