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Literature summary extracted from
- Chromium-induced tropane alkaloid production and H6H gene expression in Atropa belladonna L. (Solanaceae) in vitro-propagated plantlets (2012), Plant Physiol. Biochem., 52, 98-103.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.11 | ascorbate | required for activity | Atropa belladonna |  |
| 1.14.20.13 | ascorbate | required for activity | Atropa belladonna | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.11 | Fe2+ | required for activity | Atropa belladonna | |
| 1.14.20.13 | Fe2+ | required for activity | Atropa belladonna | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.11 | L-hyoscyamine + 2-oxoglutarate + O2 | Atropa belladonna | - |
(6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
| 1.14.20.13 | (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Atropa belladonna | - |
scopolamine + succinate + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.11 | Atropa belladonna | Q9XJ43 | collected from Sari-Garmestan of Mazandaran province in the North of Iran, gene h6h | - |
| 1.14.20.13 | Atropa belladonna | - |
collected from Sari-Garmestan of Mazandaran province in the North of Iran, gene h6h | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.11 | root | - |
Atropa belladonna | - |
| 1.14.11.11 | shoot | - |
Atropa belladonna | - |
| 1.14.20.13 | root | - |
Atropa belladonna | - |
| 1.14.20.13 | shoot | - |
Atropa belladonna | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.11 | L-hyoscyamine + 2-oxoglutarate + O2 | - |
Atropa belladonna | (6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
| 1.14.11.11 | additional information | the enzyme also catalyzes the reaction of EC 1.14.11.14 | Atropa belladonna | ? | - |
? | |
| 1.14.20.13 | (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Atropa belladonna | scopolamine + succinate + CO2 | - |
? | |
| 1.14.20.13 | additional information | the enzyme also catalyzes the reaaction of EC 1.14.11.11 | Atropa belladonna | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.11 | H6H | - |
Atropa belladonna |
| 1.14.11.11 | hyoscyamine 6beta-hydroxylase | - |
Atropa belladonna |
| 1.14.20.13 | H6H | - |
Atropa belladonna |
| 1.14.20.13 | hyoscyamine 6beta-hydroxylase | - |
Atropa belladonna |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.11.11 | Atropa belladonna | trivalent chromium, as KCr(SO4)2, induces the enzyme expression at 1 mM | up |
| 1.14.20.13 | Atropa belladonna | trivalent chromium, as KCr(SO4)2, induces the enzyme expression at 1 mM | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.11 | evolution | the enzyme is a member of the 2-oxoglutarate-dependent dioxygenase family | Atropa belladonna |
| 1.14.11.11 | metabolism | hyoscyamine 6b-hydroxylase catalyzes two consecutive oxidation reactions. The first reaction is the hydroxylation of hyoscyamine to 6beta-hydroxyhyoscyamine and the second is epoxidation of 6beta-hydroxyhyoscyamine yielding scopolamine, EC 1.14.11.14, the final metabolite in the tropane alkaloid biosynthetic pathway | Atropa belladonna |
| 1.14.11.11 | additional information | the transcript level of H6H increased under chromium treatment. This treatment also increases hyoscyamine and scopolamine contents, phenotype with decreased the growth parameters (weights and lengths of the plantlets) and chlorophyll contents and increased proline contents, overview | Atropa belladonna |
| 1.14.20.13 | evolution | the enzyme is a member of the 2-oxoglutarate-dependent dioxygenase family | Atropa belladonna |
| 1.14.20.13 | metabolism | hyoscyamine 6beta-hydroxylase catalyzes two consecutive oxidation reactions. The first reaction is the hydroxylation of hyoscyamine to 6beta-hydroxyhyoscyamine, Ec 1.14.11.11, and the second is epoxidation of 6beta-hydroxyhyoscyamine yielding scopolamine, the final metabolite in the tropane alkaloid biosynthetic pathway | Atropa belladonna |
| 1.14.20.13 | additional information | the transcript level of H6H increased under chromium treatment. This treatment also increases hyoscyamine and scopolamine contents, phenotype with decreased the growth parameters (weights and lengths of the plantlets) and chlorophyll contents and increased proline contents, overview | Atropa belladonna |
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