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Literature summary extracted from

  • Fapyane, D.; Lee, S.J.; Kang, S.H.; Lim, D.H.; Cho, K.K.; Nam, T.H.; Ahn, J.P.; Ahn, J.H.; Kim, S.W.; Chang, I.S.
    High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase alpha-subunit of Burkholderia cepacia immobilized in a carbon nanotube electrode for low glucose conditions (2013), Phys. Chem. Chem. Phys., 15, 9508-9512.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.9 additional information the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDH–menadione on a glassy carbon electrode was 10.73/s Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.9 0.4638
-
D-glucose pH and temperature not specified in the publication Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 18000
-
1 * 59800 + 1 * 18000, SDS-PAGE Burkholderia cepacia
1.1.5.9 59800
-
1 * 59800 + 1 * 18000, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.9 D-glucose + a quinone Burkholderia cepacia
-
D-glucono-1,5-lactone + a quinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Burkholderia cepacia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 recombinant enzyme from Escherichia coli strain BL21RIL Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + a quinone
-
Burkholderia cepacia D-glucono-1,5-lactone + a quinol
-
?
1.1.5.9 D-glucose + menadione
-
Burkholderia cepacia D-glucono-1,5-lactone + menadiol
-
?
1.1.5.9 D-glucose + oxidized 2,6-dichlorophenol indophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 dimer 1 * 59800 + 1 * 18000, SDS-PAGE Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD-dependent glucose dehydrogenase
-
Burkholderia cepacia
1.1.5.9 FAD-GDH
-
Burkholderia cepacia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.9 69.54
-
D-glucose pH and temperature not specified in the publication Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD dependent on, bound in the catalytic site Burkholderia cepacia