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Literature summary extracted from

  • Reis, M.; Alves, C.N.; Lameira, J.; Tunon, I.; Marti, S.; Moliner, V.
    The catalytic mechanism of glyceraldehyde 3-phosphate dehydrogenase from Trypanosoma cruzi elucidated via the QM/MM approach (2013), Phys. Chem. Chem. Phys., 15, 3772-3785.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.12 molecular dynamics simulations based on PDB entry 1K3T. The first stage of the reaction (oxidoreduction) takes place in the Pi site (energetically more favourable), with the formation of oxyanion thiohemiacetal and thioacylenzyme intermediates without acid-base assistance of His194. Residue Arg249 has an important role in the ability of the enzyme to bind the glyceraldehyde 3-phosphatesubstrate, which interacts with NAD+ and other important residues, such as Cys166, Glu109, Thr167, Ser247 and Thr226, in the GAPDH active site Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 (3-[(4-nitrophenoxy)carbonyl]but-3-en-1-yl)phosphonic acid
-
 Trypanosoma cruzi
1.2.1.12 3-(p-nitrophenoxycarboxyl)-3-ethylene propyl dihydroxyphosphinate propyl dihydroxyphosphonate analogue of substrate glyceraldehyde 3-phosphate. The energy profiles correspond to the nucleophilic attack of Cys166 on the atom C1 of the carbonyl group of the inhibitor. The barrier for the inhibition reaction is lower than that observed for a natural substrate Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ Trypanosoma cruzi
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Trypanosoma cruzi
-
-
-
1.2.1.12 Trypanosoma cruzi P22513
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Trypanosoma cruzi 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.12 GAPDH
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Trypanosoma cruzi