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Literature summary extracted from

  • Debieu, D.; Bach, J.; Montesinos, E.; Fillinger, S.; Leroux, P.
    Role of sterol 3-ketoreductase sensitivity in susceptibility to the fungicide fenhexamid in Botrytis cinerea and other phytopathogenic fungi (2013), Pest Manag. Sci., 69, 642-651.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.270 additional information erg27 mutation and erg27 polymorphism in different strains and fenhexamid phenotypes, overview Botrytis cinerea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.270 acetonitrile
-
Botrytis cinerea
1.1.1.270 dimethyl sulfoxide
-
Botrytis cinerea
1.1.1.270 ethanol
-
Botrytis cinerea
1.1.1.270 fenhexamid Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase. Sterol 3-ketoreductase sensitivity to fenhexamid in the various fungal strains, overview Botrytis cinerea
1.1.1.270 propan-2-ol
-
Botrytis cinerea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.270 microsome
-
Botrytis cinerea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.270 Mg2+ required Botrytis cinerea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.270 Botrytis cinerea
-
gene erg27
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.270 mycelium
-
Botrytis cinerea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.270 4alpha-methylfecosterone + NADPH + H+
-
Botrytis cinerea 4alpha-methylfecosterol + NADP+ 4alpha-methylfecosterol is further converted to ergosterol ?
1.1.1.270 fecosterone + NADPH + H+
-
Botrytis cinerea fecosterol + NADP+
-
?
1.1.1.270 additional information substrate for 3-ketoreductase in the first sterol C-4 demethylation cycle is 4alpha-methylfecosterone, whereas that for the second C-4 demethylation cycle is fecosterone Botrytis cinerea ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.270 sterol 3-ketoreductase
-
Botrytis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.270 30
-
assay at Botrytis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.270 7
-
assay at Botrytis cinerea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.270 NADPH
-
Botrytis cinerea

General Information

EC Number General Information Comment Organism
1.1.1.270 malfunction two Botrytis cinerea fenhexamid-resistant phenotypes both result from mutations in the erg27 gene encoding 3-ketoreductase. Erg27 mutations causing amino acid substitutions in or near the transmembrane domain strongly decrease the affinity of fenhexamid for 3-ketoreductase Botrytis cinerea
1.1.1.270 metabolism the 3-ketoreductase is involved at C-4 demethylation steps during ergosterol biosynthesis. Steryl compound contents of Botrytis strains with different fenhexamid susceptibilities, overview Botrytis cinerea