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Literature summary extracted from

  • Yadav, S.S.; Srikanth, E.; Singh, N.; Rathaur, S.
    Identification of GR and TrxR systems in Setaria cervi: Purification and characterization of glutathione reductase (2013), Parasitol. Int., 62, 193-198.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 2,4-dihydroxybenzylamine specific inhibitor, complete inhibition at 0.001 mM Setaria cervi
1.8.1.7 diethyldicarbonate
-
 Setaria cervi
1.8.1.7 N-Acetylimidazole
-
 Setaria cervi
1.8.1.7 N-ethylmaleimide
-
 Setaria cervi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.27
-
 NADPH in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi
1.8.1.7 0.59
-
 glutathione disulfide in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 50000
-
 2 * 50000, SDS-PAGE Setaria cervi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+ Setaria cervi
-
 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Setaria cervi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 2',5'-ADP agarose affinity chromatography and DEAE Sephadex gel filtration Setaria cervi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 adult
-
 Setaria cervi
-
1.8.1.7 larva
-
 Setaria cervi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 7.16
-
 homogenate, in 50 mM potassium phosphate buffer pH 7.0, at 25°C Setaria cervi
1.8.1.7 643
-
 89.8fold purified enzyme, in 50 mM potassium phosphate buffer pH 7.0, at 25°C Setaria cervi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Setaria cervi glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 homodimer 2 * 50000, SDS-PAGE Setaria cervi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 35
-
-
 Setaria cervi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7
-
-
 Setaria cervi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 flavin
-
 Setaria cervi
1.8.1.7 NADPH
-
 Setaria cervi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.7 0.27
-
 in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi diethyldicarbonate
1.8.1.7 0.5
-
 in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi N-ethylmaleimide
1.8.1.7 0.71
-
 in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi N-Acetylimidazole