Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ouchi, T.; Tomita, T.; Horie, A.; Yoshida, A.; Takahashi, K.; Nishida, H.; Lassak, K.; Taka, H.; Mineki, R.; Fujimura, T.; Kosono, S.; Nishiyama, C.; Masui, R.; Kuramitsu, S.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
    Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus (2013), Nat. Chem. Biol., 9, 277-283.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.B19 expression in Escherichia coli Sulfolobus acidocaldarius
6.3.2.B19 expression in Escherichia coli Sulfurisphaera tokodaii
6.3.2.43 expression in Escherichia coli Sulfolobus acidocaldarius

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.B19 X-ray diffraction with resolution of 1.8 A of the enzyme complexed with LysW, L-glutamate, ADP, Mg2+, Zn2+ and sulfate. X-ray diffraction with resolution of 1.87 A of the enzyme complexed with ADP Sulfurisphaera tokodaii
6.3.2.43 crystal structures of the AMP-PNP–bound complex of LysX Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.43 N264G/T265T the mutant shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.B19 31534
-
 4 * 31534, calculated from sequence Sulfurisphaera tokodaii
6.3.2.B19 31715
-
 x * 31715, calculated from sequence Sulfolobus acidocaldarius
6.3.2.B19 126200
-
 mass spectrometry Sulfurisphaera tokodaii
6.3.2.B19 146000
-
 analytical ultracentrifugation Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfurisphaera tokodaii
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfolobus acidocaldarius arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfolobus acidocaldarius DSM 639 arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfurisphaera tokodaii 7
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate Sulfolobus acidocaldarius lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate Sulfolobus acidocaldarius DSM 639 lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.B19 Sulfolobus acidocaldarius Q4J8E7
-
-
6.3.2.B19 Sulfolobus acidocaldarius DSM 639 Q4J8E7
-
-
6.3.2.B19 Sulfurisphaera tokodaii Q970U6
-
-
6.3.2.B19 Sulfurisphaera tokodaii 7 Q970U6
-
-
6.3.2.43 Sulfolobus acidocaldarius Q4JAP9
-
-
6.3.2.43 Sulfolobus acidocaldarius DSM 639 Q4JAP9
-
-
6.3.2.43 Thermus thermophilus Q5SH23
-
-
6.3.2.43 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SH23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.B19 recombinant enzyme Sulfolobus acidocaldarius
6.3.2.43 recombinant enzyme Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfurisphaera tokodaii ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.B19 ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfurisphaera tokodaii 7 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate mutant enzyme N264G/T265T shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate mutant enzyme N264G/T265T shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.B19 ? x * 31715, calculated from sequence Sulfolobus acidocaldarius
6.3.2.B19 tetramer 4 * 31534, calculated from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
6.3.2.B19 ArgX
-
 Sulfolobus acidocaldarius
6.3.2.B19 Saci_1621 locus name Sulfolobus acidocaldarius
6.3.2.B19 StArgX
-
 Sulfurisphaera tokodaii
6.3.2.B19 STK_15050
-
 Sulfurisphaera tokodaii
6.3.2.43 LysX
-
 Sulfolobus acidocaldarius
6.3.2.43 Saci_0754 locus name Sulfolobus acidocaldarius
6.3.2.43 TtLysX
-
 Thermus thermophilus

General Information

EC Number General Information Comment Organism
6.3.2.B19 malfunction no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible Sulfolobus acidocaldarius
6.3.2.B19 physiological function arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius
6.3.2.43 malfunction no growth of deletion mutant without L-lysine. Growth is possible with lysine supplementation. A 0.05-mM concentration of lysine is most effective to support the growth of the deletion mutant, but a higher concentration of lysine decreases the growth rate Sulfolobus acidocaldarius
6.3.2.43 physiological function lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius